Zhao Lehan, Lin Xianfeng, Duan Nuo, Mahmood Khan Imran, Wang Zhouping, Wu Shijia
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
Food Chem. 2023 Jun 1;410:135425. doi: 10.1016/j.foodchem.2023.135425. Epub 2023 Jan 7.
Malachite green (MG), as a parasiticide, is widely used in aquaculture to increase the production of the fishery industry. It poses a great danger to both the food system and the human body. In this study, a one-pot reverse microemulsion polymerization was employed to combine the gold nanoclusters (AuNCs) with molecularly imprinted polymers (MIPs) and covalent organic frameworks (COFs) to synthesize an efficient fluorescent hybrid probe (AuNCs@COFs@MIPs) for selective detection of MG. The specific recognition of AuNCs@COFs@MIPs towards MG triggers the fluorescence quenching of AuNCs. The fluorescent response was linearly related to the concentration over the range of 10-150 nmol/L with a limit of detection of 2.78 nmol/L. In addition, the proposed probe was further applied to fish and water samples. A favorable recovery ranged from 97.34 to 101.51 % toward trace amounts of MG indicating its promising application for detecting residue of veterinary drugs.
孔雀石绿（MG）作为一种杀虫剂，在水产养殖中被广泛使用以提高渔业产量。它对食品系统和人体都构成了巨大威胁。在本研究中，采用一锅法反向微乳液聚合将金纳米簇（AuNCs）与分子印迹聚合物（MIPs）和共价有机框架（COFs）相结合，合成了一种用于选择性检测MG的高效荧光杂交探针（AuNCs@COFs@MIPs）。AuNCs@COFs@MIPs对MG的特异性识别引发了AuNCs的荧光猝灭。荧光响应在10 - 150 nmol/L范围内与浓度呈线性关系，检测限为2.78 nmol/L。此外，所提出的探针进一步应用于鱼和水样。对痕量MG的回收率良好，范围为97.34%至101.51%，表明其在检测兽药残留方面具有广阔的应用前景。
