Wei Guo, Zhang Yimeng, Wang Rui, Wang Chuanliang, Geng Xuhui, Zhang Peng, Li Jinhua, Chen Lingxin, Song Zhihua
School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai, 264005, People's Republic of China.
Department of Instrumentation & Analytical Chemistry, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Key Laboratory of Deep-Sea Composition Detection Technology of Liaoning Province, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
Mikrochim Acta. 2025 Aug 29;192(9):626. doi: 10.1007/s00604-025-07465-7.
CdTe QD-doped MIP-based TLC combined with a miniaturized fluorimeter system (namely the system) was developed to simultaneously detect malachite green (4-[(4-(dimethylamino)phenyl)(phenyl)methyl]-N,N-dimethylbenzenaminium chloride, MG) and crystal violet (4-(Bis{4-(dimethylamino)phenyl}methylidene)-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride, CV). The system integrated CdTe quantum dots-based molecularly imprinted polymers nanoparticles (CdTe-MIP/SiO NPs) with thin layer chromatography (TLC) plates. Firstly, CdTe-MIP/SiO NPs were synthesized by forming an imprinted layer on silica nanoparticle surfaces through a simple one-pot reaction. Then, the system was coupled with a miniaturized fluorimeter. Thus, the good optical properties of CdTe QDs (satisfactory fluorescence quantum yield, high fluorescence intensity, appropriate emission and excitation spectrums, good water solubility and photostability, tunability, etc.), specific recognition properties of MIPs toward template molecules and the related compounds, classical performances of TLC, and high sensitivity of the miniaturized fluorimeter were combined. The fluorescence intensity of the system exhibited linear quenching relationships with MG and CV concentrations within 0.01 ~ 1 μmol/L and 1 ~ 20 μmol/L, respectively. The detection limits for MG and CV were determined to be 8.5 nmol/L and 8.2 nmol/L, separately. Furthermore, the system successfully determined MG and CV in five kinds of fish samples within 10 min, with recoveries ranging from 91.9% to 114.6% for MG and 86.4% to 118.9% for CV. Validation by conventional HPLC showed that the system was suitable for the simultaneous determination of MG and CV. Therefore, the system is hopeful for extensive applications in real samples analysis.
开发了基于碲化镉量子点掺杂分子印迹聚合物的薄层色谱法（TLC）与小型荧光计系统（即该系统）相结合的方法，用于同时检测孔雀石绿（4-[(4-(二甲基氨基)苯基)(苯基)甲基]-N,N-二甲基苯铵氯化物，MG）和结晶紫（4-(双{4-(二甲基氨基)苯基}亚甲基)-N,N-二甲基环己-2,5-二烯-1-亚胺氯化物，CV）。该系统将基于碲化镉量子点的分子印迹聚合物纳米颗粒（CdTe-MIP/SiO NPs）与薄层色谱（TLC）板集成在一起。首先，通过简单的一锅法反应在二氧化硅纳米颗粒表面形成印迹层来合成CdTe-MIP/SiO NPs。然后，该系统与小型荧光计耦合。因此，可以结合碲化镉量子点良好的光学性质（令人满意的荧光量子产率、高荧光强度、合适的发射和激发光谱、良好的水溶性和光稳定性、可调节性等）、分子印迹聚合物对模板分子及相关化合物的特异性识别性质以及薄层色谱的经典性能和小型荧光计的高灵敏度。该系统的荧光强度分别在0.01 ~ 1 μmol/L和1 ~ 20 μmol/L范围内与MG和CV浓度呈现线性猝灭关系。MG和CV的检测限分别确定为8.5 nmol/L和8.2 nmol/L。此外，该系统在10分钟内成功测定了五种鱼类样品中的MG和CV，MG的回收率为91.9%至114.6%，CV的回收率为86.4%至118.9%。通过传统高效液相色谱法验证表明该系统适用于同时测定MG和CV。因此，该系统有望在实际样品分析中得到广泛应用。
