Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518000, China; Center for Clinical Precision Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China; School of Clinical Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China.
Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong Special Administrative Region; Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518000, China.
Int J Med Microbiol. 2023 Jan;313(1):151573. doi: 10.1016/j.ijmm.2023.151573. Epub 2023 Jan 6.
Uropathogenic Escherichia coli (UPEC) are causative agent that causes urinary tract infections (UTIs) and the recent emergence of multidrug resistance (MDR) of UPEC increases the burden on the community. Recent studies of bacterial outer membrane vesicles (OMV) identified various factors including proteins, nucleic acids, and small molecules which provided inter-cellular communication within the bacterial population. However, the components of UPEC-specific OMVs and their functional role remain unclear. Here, we systematically determined the proteomes of UPEC-OMVs and identified the specific components that provide functions to the recipient bacteria. Based on the functional network of OMVs' proteomes, a group of signaling peptides was found in all OMVs which provide communication among bacteria. Moreover, we demonstrated that treatment with UPEC-OMVs affected the motility and biofilm formation of the recipient bacteria, and further identified aromatic amino acid (AAA) biosynthesis proteins as the key factors to provide their movement.
尿路致病性大肠杆菌（UPEC）是引起尿路感染（UTIs）的病原体，而 UPEC 的多药耐药性（MDR）的最近出现增加了社区的负担。最近对细菌外膜囊泡（OMV）的研究鉴定了各种因子，包括蛋白质、核酸和小分子，这些因子在细菌种群内提供了细胞间的通讯。然而，UPEC 特异性 OMV 的组成及其功能作用仍然不清楚。在这里，我们系统地确定了 UPEC-OMV 的蛋白质组，并鉴定了为受体细菌提供功能的特定成分。基于 OMV 蛋白质组的功能网络，发现所有 OMV 中都存在一组信号肽，这些信号肽在细菌之间提供通讯。此外，我们证明了用 UPEC-OMV 处理会影响受体细菌的运动性和生物膜形成，并进一步确定芳香族氨基酸（AAA）生物合成蛋白是提供运动的关键因素。
