You Min, Zhang Long, Ding Junxiao
Department of Dermatology, Affiliated Hospital of Gansu Medical College, Pingliang, Gansu, People's Republic of China.
Clin Cosmet Investig Dermatol. 2023 Jan 5;16:39-46. doi: 10.2147/CCID.S378985. eCollection 2023.
The aim of this study was to investigate the diagnostic ability and prognostic value of miR-342-3p in SLE patients by detecting the expression level of serum miR-342-3p in SLE patients.
The expression level of serum miR-342-3p in all subjects was determined by qRT-PCR technology, and the correlation of miR-342-3p with SLEDAI scores was evaluated using Spearman correlation coefficient. The diagnostic value of miR-342-3p in SLE was assessed using ROC curve. The prognostic value of miR-342-3p in SLE patients was analyzed by Kaplan-Meier survival curve and multivariate COX regression.
The qRT-PCR results showed that the expression level of serum miR-342-3p in SLE patients was significantly lower than that in healthy controls ( < 0.001). Serum miR-342-3p expression level was negatively correlated with SLEDAI scores (r = - 0.810, < 0.001). The ROC curve suggested that serum miR-342-3p expression level was discriminative between SLE patients and healthy controls. Survival analysis results indicated that SLE patients with low serum miR-342-3p expression had a higher probability of poor prognosis of SLE (Log rank = 0.003).
The expression level of serum miR-342-3p was valuable in the diagnosis of SLE. Meanwhile, the low expression level of serum miR-342-3p was associated with the poor prognosis of SLE.
本研究旨在通过检测系统性红斑狼疮（SLE）患者血清中miR - 342 - 3p的表达水平，探讨其在SLE患者中的诊断能力及预后价值。
采用qRT - PCR技术测定所有受试者血清中miR - 342 - 3p的表达水平，并使用Spearman相关系数评估miR - 342 - 3p与SLE疾病活动指数（SLEDAI）评分的相关性。采用ROC曲线评估miR - 342 - 3p在SLE中的诊断价值。通过Kaplan - Meier生存曲线和多因素COX回归分析miR - 342 - 3p在SLE患者中的预后价值。
qRT - PCR结果显示，SLE患者血清中miR - 342 - 3p的表达水平显著低于健康对照（<0.001）。血清miR - 342 - 3p表达水平与SLEDAI评分呈负相关（r = - 0.810，<0.001）。ROC曲线表明，血清miR - 342 - 3p表达水平可区分SLE患者和健康对照。生存分析结果表明，血清miR - 342 - 3p表达水平低的SLE患者发生SLE预后不良的概率较高（Log秩 = 0.003）。
血清miR - 342 - 3p表达水平对SLE诊断具有重要价值。同时，血清miR - 342 - 3p低表达与SLE预后不良相关。
