Tang Zhao Ming, Fang Min, Wang Jin Ping, Cai Peng Cheng, Wang Ping, Hu Li Hua
Department of Laboratory Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Clin Lab Anal. 2014 Nov;28(6):446-51. doi: 10.1002/jcla.21708. Epub 2014 Mar 22.
Plasma miR-21 is widely investigated as biomarker in many diseases. Recent studies show that miR-21 participates in the development of systemic lupus erythematosus (SLE). The aim of this study was to evaluate the expression profile of miR-21 in the plasma of SLE patients.
Relative quantities of plasma miR-21 both in SLE patients and healthy controls were determined by relative qRT-PCR under endogenous and exogenous controls. The diagnostic value of plasma miR-21 was evaluated in SLE patients. Data of some SLE-associated clinical parameters were collected.
Eighty participants from Central China were recruited. Forty-four participants were new-onset SLE patients and the others were healthy controls. Plasma miR-21 level in SLE patients was higher than that of healthy controls (P = 0.031). Receiver operating characteristic analysis of plasma miR-21 revealed an Area Under Curve (AUC) of 0.64 ± 0.06 (95% CI: 0.51-0.76, P = 0.03854) when differentiating SLE from healthy controls. The level of plasma miR-21 was not associated with the level of white blood cells (P = 0.4284), red blood cells (P = 0.4079), and platelets (P = 0.4961), but significantly correlated with the level of plasma complement C3 (r = -0.5297, P = 0.0004), C4 (r = -0.4732, P = 0.0020), and serum uric acid (r = 0.3932, P = 0.0121) in SLE patients.
Plasma miR-21 in SLE patients from Central China is overexpressed. Since circulating miR-21 is aberrantly expressed in many diseases, the applying of it as a disease biomarker should be considered carefully.
血浆miR-21作为许多疾病的生物标志物受到广泛研究。近期研究表明，miR-21参与系统性红斑狼疮（SLE）的发病过程。本研究旨在评估SLE患者血浆中miR-21的表达谱。
在内源性和外源性对照下，通过相对定量逆转录聚合酶链反应（qRT-PCR）测定SLE患者和健康对照者血浆中miR-21的相对含量。评估血浆miR-21在SLE患者中的诊断价值。收集一些与SLE相关的临床参数数据。
招募了来自中国中部地区的80名参与者。其中44名参与者为新发病的SLE患者，其余为健康对照者。SLE患者血浆miR-21水平高于健康对照者（P = 0.031）。血浆miR-21的受试者工作特征分析显示，在区分SLE与健康对照时，曲线下面积（AUC）为0.64±0.06（95%可信区间：0.51 - 0.76，P = 0.03854）。血浆miR-21水平与白细胞水平（P = 0.4284）、红细胞水平（P = 0.4079）和血小板水平（P = 0.4961）无关，但与SLE患者血浆补体C3水平（r = -0.5297，P = 0.0004）、C4水平（r = -0.4732，P = 0.0020）和血清尿酸水平（r = 0.3932，P = 0.0121）显著相关。
中国中部地区SLE患者血浆miR-21表达上调。由于循环miR-21在许多疾病中表达异常，将其作为疾病生物标志物的应用应谨慎考虑。
