Department of Clinical Laboratory, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Department of Clinical Laboratory, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Clin Biochem. 2022 Sep;107:55-61. doi: 10.1016/j.clinbiochem.2022.05.004. Epub 2022 May 19.
To explore the clinical value of miR-124-3p and miR-377-3p in plasma, and to examine whether these microRNAs can serve as biomarkers for systemic lupus erythematosus.
Samples from 50 patients with SLE and 47 healthy individuals were collected from the Rheumatology and Immunology Department at the Second Affiliated Hospital of Chongqing Medical University. The expression of miR-124-3p and miR-377-3p in peripheral blood mononuclear cells (PBMCs) and serum were determined by quantitative reverse-transcription PCR (RT-PCR). We then further analyzed the associations between these microRNAs and clinically relevant indicators. We employed receiver operating characteristic (ROC) curves and the area under the ROC curves (AUCs) to validate the diagnostic value of miR-124-3p and miR-377-3p.
The expression of miR-124-3p and miR-377-3p was significantly upregulated in PBMCs and serum from SLE patients compared with the normal control group (P < 0.05). ROC curve analysis indicated that plasma miR-124-3p and miR-377-3p were thus candidate diagnostic biomarkers for SLE, with AUCs of 0.714 (95% CI, 0.610 to 0.820, P < 0.05) and 0.705 (95% CI, 0.600 to 0.809, P < 0.05), respectively. Furthermore, the combined diagnostic efficiency of miR-124-3p and miR-377-3p was higher than that for miR-124-3p or miR-377-3p alone, and the AUCs for miR-124-3p and miR-377-3p in plasma were higher than in PBMCs. Plasma miR-124-3p expression was also associated with various clinicopathological parameters such as antiC1q and C3. Binary logistic regression analysis revealed that the expression of miR-377-3p was an independent predictor for SLE, and the plasma miR-124-3p was independently associated with the remission rate of SLE.
Circulating miR-124-3p and miR-377-3p may constitute promising biomarkers for the diagnosis of SLE.
探讨血浆中 miR-124-3p 和 miR-377-3p 的临床价值，并研究这些 microRNAs 是否可作为系统性红斑狼疮的生物标志物。
收集来自重庆医科大学附属第二医院风湿免疫科的 50 例系统性红斑狼疮患者和 47 例健康个体的样本。采用实时定量逆转录 PCR（qRT-PCR）检测外周血单个核细胞（PBMC）和血清中 miR-124-3p 和 miR-377-3p 的表达。进一步分析这些 microRNAs 与临床相关指标之间的关系。采用受试者工作特征（ROC）曲线和曲线下面积（AUC）验证 miR-124-3p 和 miR-377-3p 的诊断价值。
与正常对照组相比，SLE 患者的 PBMC 和血清中 miR-124-3p 和 miR-377-3p 的表达显著上调（P<0.05）。ROC 曲线分析表明，血浆 miR-124-3p 和 miR-377-3p 可作为 SLE 的候选诊断生物标志物，AUC 分别为 0.714（95%CI，0.610 至 0.820，P<0.05）和 0.705（95%CI，0.600 至 0.809，P<0.05）。此外，miR-124-3p 和 miR-377-3p 的联合诊断效率高于 miR-124-3p 或 miR-377-3p 单独检测，且血浆 miR-124-3p 和 miR-377-3p 的 AUC 高于 PBMCs。血浆 miR-124-3p 的表达还与抗 C1q 和 C3 等多种临床病理参数相关。二元逻辑回归分析显示，miR-377-3p 的表达是 SLE 的独立预测因子，而血浆 miR-124-3p 与 SLE 的缓解率独立相关。
循环 miR-124-3p 和 miR-377-3p 可能是 SLE 诊断有前途的生物标志物。
