Department of Molecular Biotechnology, Graduate School of Health Sciences, Aydin Adnan Menderes University, Aydin, Turkey.
Department of Histology and Embryology, School of Medicine, Aydin Adnan Menderes University, Aydin, Turkey.
J Obstet Gynaecol. 2022 Nov;42(8):3568-3576. doi: 10.1080/01443615.2022.2158318. Epub 2023 Jan 13.
In this study, the effect of applying wharton jelly mesenchymal stromal cells (WJ-MSC) isolated from the human umbilical cord tissue on the neonatal mouse model caused experimental asphyxia in mice was investigated. WJ-MSC surface markers (CD44, CD90, CD105) were characterised by immunofluorescence staining, and pluripotency genes (Nanog, Oct-4, Sox-2) were characterised by qPCR. Blood, prefrontal cortex, cerebellum, hippocampus, lung, heart, kidney, and liver tissues were analysed twenty days after subcutaneously administered WJ-MSC. WJ-MSC administration significantly decreased serum TNF-α, NSE, GFAP, and IL-6 levels in the asphyxia mice. It was determined that WJ-MSC application in tissues accelerated cell regeneration and decreased oxidative stress. In conclusion, this study showed that multiorgan damage in asphyxia could be prevented by applying WJ-MSC at an early stage. Therefore, WJ-MSC application in infants with neonatal asphyxia in the clinic may be an innovative method in the future.
本研究探讨了从人脐带组织中分离的牙髓间充质基质细胞（WJ-MSC）对实验性窒息新生小鼠模型的影响。通过免疫荧光染色对 WJ-MSC 表面标志物（CD44、CD90、CD105）进行了特征描述，并通过 qPCR 对多能基因（Nanog、Oct-4、Sox-2）进行了特征描述。在皮下给予 WJ-MSC 二十天后，分析了血液、前额叶皮层、小脑、海马、肺、心脏、肾脏和肝脏组织。WJ-MSC 给药可显著降低窒息小鼠血清 TNF-α、NSE、GFAP 和 IL-6 水平。结果表明，WJ-MSC 在组织中的应用加速了细胞再生，降低了氧化应激。总之，本研究表明，早期应用 WJ-MSC 可预防窒息引起的多器官损伤。因此，在临床上对新生儿窒息的婴儿应用 WJ-MSC 可能是未来的一种创新方法。
