Rubin B Y, Blamire J
Mol Gen Genet. 1978 Nov 29;167(2):177-84. doi: 10.1007/BF00266911.
A single recessive nuclear gene mutation has been isolated from strain 123.1C of Saccharomyces cerevisiae which appears to be conditionally deficient in nuclear DNA metabolism. Growth of the mutant strain at the elevated temperature of 36 degree C results in rapid loss of cell viability. However, no apparent reduction in the rate of radioisotope incorporation into DNA was detected during this period. When haploid cells carrying this temperature sensitive lesion were exposed to the restrictive temperature for varying lengths of time, returned to the permissive temperature, mated with a non-temperature sensitive strain and then the resulting diploids made to undergo meiosis, a greatly reduced number of viable spores were produced. Genetic analysis of the viable spores produced by these diploids has revealed aberrant auxotrophic marker segregation patterns. Thus, these results suggest that the mutated gene hardbored in this strain plays a vital role in the metabolism of the nuclear genome.
从酿酒酵母123.1C菌株中分离出一个单隐性核基因突变，该突变似乎在核DNA代谢方面存在条件性缺陷。突变菌株在36℃的高温下生长会导致细胞活力迅速丧失。然而，在此期间未检测到放射性同位素掺入DNA的速率有明显降低。当携带这种温度敏感损伤的单倍体细胞在限制温度下暴露不同时间，再回到允许温度，与非温度敏感菌株交配，然后使产生的二倍体进行减数分裂时，产生的存活孢子数量大大减少。对这些二倍体产生的存活孢子进行遗传分析，发现了异常的营养缺陷型标记分离模式。因此，这些结果表明，该菌株中携带的突变基因在核基因组的代谢中起着至关重要的作用。
