Rubin B Y, Blamire J
Mol Gen Genet. 1977 Nov 4;156(1):41-7. doi: 10.1007/BF00272250.
A single nuclear gene mutation has been isolated from strain 123.1C of Saccharomyces cerevisiae which is conditionally deficient in mitochondrial DNA metabolism. Growth of the haploid in media containing dextrose, a repressing carbon source, at 36 degrees C causes the rapid cessation of mitochondrial DNA synthesis as analyzed by radioactive 3H-adenine incorporation into mitochondrial DNA. These cells continue to grow and divide giving rise to neutral petites which are devoid of mitochondrial DNA as measured by radioactive incorporation of 3H-adenine at the permissive temperature. Growth of the haploid cells in media containing glycerol, a non-repressing carbon source, at 36 degrees C does not prevent mitochondrial DNA synthesis, however, the population of cells becomes partially petite. When such petites are analyzed, they are found to be suppressive and to contain mitochondrial DNA as measured in the manner described above. The action of this mutated gene appears to involve the sunthetic aspects of mitochondrial DNA metabolism, as haploid cells prelabeled in dextrose media with 3H-adenine show no loss or degradation of mitochondrial DNA at the restrictive temperature of 36 degrees C.
从酿酒酵母123.1C菌株中分离出一种单细胞核基因突变体，该菌株在线粒体DNA代谢方面存在条件性缺陷。在含有葡萄糖（一种抑制性碳源）的培养基中，于36℃培养单倍体时，通过将放射性3H-腺嘌呤掺入线粒体DNA进行分析，发现线粒体DNA合成迅速停止。这些细胞继续生长和分裂，产生中性小菌落，在允许温度下通过3H-腺嘌呤的放射性掺入测量，这些小菌落不含线粒体DNA。在含有甘油（一种非抑制性碳源）的培养基中，于36℃培养单倍体细胞，不会阻止线粒体DNA合成，然而，细胞群体部分变为小菌落。当分析这些小菌落时，发现它们具有抑制性，并且如上所述测量时含有线粒体DNA。该突变基因的作用似乎涉及线粒体DNA代谢的合成方面，因为在葡萄糖培养基中用3H-腺嘌呤预标记的单倍体细胞在36℃的限制温度下未显示线粒体DNA的损失或降解。
