Steno Diabetes Center Aarhus, Aarhus University Hospital, 8200 Aarhus, Denmark; Hormonal and Bone Diseases, Aarhus University Hospital, 8200 Aarhus, Denmark; Department of Clinical Pharmacology, Aarhus University Hospital, 8200 Aarhus, Denmark.
Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark; Research Laboratory for Biochemical Pathology, Department of Clinical Medicine, Aarhus University, 8200 Aarhus, Denmark; Steno Diabetes Center Aarhus, Aarhus University Hospital, 8200 Aarhus, Denmark; Department of Nuclear Medicine & PET-Centre, Aarhus University Hospital, 8200 Aarhus, Denmark.
STAR Protoc. 2023 Mar 17;4(1):102008. doi: 10.1016/j.xpro.2022.102008. Epub 2023 Jan 12.
The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies. For complete details on the use and execution of this protocol, please refer to Farup et al. (2021)..
干细胞激活和分化的能力对于维持骨骼肌的再生能力至关重要。在这里，我们详细介绍了使用荧光激活细胞分选术对原代人成纤维脂肪祖细胞和骨骼肌干细胞进行特异性定量和分离的步骤。我们描述了所分离细胞群体的重要表型特征，例如进入细胞周期的时间和细胞分化评估。该技术已应用于手术和针吸活检获得的组织。有关该方案使用和执行的完整详细信息，请参考 Farup 等人。(2021)。
