Manokawinchoke Jeeranan, Chareonvit Suconta, Trachoo Vorapat, Limraksasin Phoonsuk, Egusa Hiroshi, Osathanon Thanaphum
Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
J Dent Sci. 2023 Jan;18(1):105-111. doi: 10.1016/j.jds.2022.07.003. Epub 2022 Jul 15.
BACKGROUND/PURPOSE: Mechanical force differentially regulates periodontal ligament functions depending on types, magnitudes, and duration of stimulation. Intermittent compressive force (ICF) promotes an mineralization in human periodontal ligament cells. The present study investigated the effect of ICF on dentin matrix protein-1 (DMP1) expression in human periodontal ligament stem cells (hPDLSCs).
Cells were treated with ICF in a serum-free culture medium for 24 h The mRNA and protein expression were examined using real-time polymerase chain reaction, immunofluorescence staining and Western blot analysis, respectively.
The exposure to ICF in a serum-free condition significantly induced DMP1 expression in both mRNA and protein levels. The effect of ICF-induced expression was inhibited by pretreatment with cycloheximide, indicating the requirement of the intermediated molecule(s). Pretreatment with transforming growth factor β (TGF-β) receptor inhibitor (SB431542) or neutralized antibody against TGF-β1 prior to ICF application abolished the effect of ICF-induced expression. Further, recombinant TGF-β1 treatment stimulated expression.
The present study illustrated that ICF induces expression in hPDLSCs via the regulation of TGF-β signaling pathway.
背景/目的：机械力根据刺激的类型、大小和持续时间对牙周膜功能进行不同的调节。间歇性压缩力（ICF）可促进人牙周膜细胞矿化。本研究调查了ICF对人牙周膜干细胞（hPDLSCs）中牙本质基质蛋白-1（DMP1）表达的影响。
在无血清培养基中用ICF处理细胞24小时，分别使用实时聚合酶链反应、免疫荧光染色和蛋白质印迹分析检测mRNA和蛋白质表达。
在无血清条件下暴露于ICF可显著诱导DMP1在mRNA和蛋白质水平的表达。用环己酰亚胺预处理可抑制ICF诱导的表达，表明需要中间分子。在施加ICF之前，用转化生长因子β（TGF-β）受体抑制剂（SB431542）或抗TGF-β1中和抗体预处理可消除ICF诱导表达的作用。此外，重组TGF-β1处理可刺激表达。
本研究表明ICF通过调节TGF-β信号通路诱导hPDLSCs中表达。
