人类基因组的遗传连锁图谱。

A genetic linkage map of the human genome.

作者信息

Donis-Keller H, Green P, Helms C, Cartinhour S, Weiffenbach B, Stephens K, Keith T P, Bowden D W, Smith D R, Lander E S

机构信息

Department of Human Genetics, Collaborative Research, Inc., Bedford, Massachusetts 01730.

出版信息

Cell. 1987 Oct 23;51(2):319-37. doi: 10.1016/0092-8674(87)90158-9.

DOI:10.1016/0092-8674(87)90158-9

PMID:3664638

Abstract

We report the construction of a linkage map of the human genome, based on the pattern of inheritance of 403 polymorphic loci, including 393 RFLPs, in a panel of DNAs from 21 three-generation families. By a combination of mathematical linkage analysis and physical localization of selected clones, it was possible to arrange these loci into linkage groups representing 23 human chromosomes. We estimate that the linkage map is detectably linked to at least 95% of the DNA in the human genome.

摘要

我们报告了人类基因组连锁图谱的构建情况，该图谱基于来自21个三代家庭的一组DNA中403个多态性位点（包括393个限制性片段长度多态性，RFLP）的遗传模式。通过数学连锁分析和选定克隆的物理定位相结合的方法，有可能将这些位点排列成代表23条人类染色体的连锁群。我们估计该连锁图谱与人类基因组中至少95%的DNA可检测地连锁。