Zarbl H, Latreille J, Jolicoeur P
Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Quebec, Canada.
Cell. 1987 Nov 6;51(3):357-69. doi: 10.1016/0092-8674(87)90632-5.
Morphologic revertants of FBJ murine sarcoma virus (v-fos)-transformed rat-1 fibroblasts were isolated using a novel selection procedure based on prolonged retention of rhodamine 123 within mitochondria of v-fos-transformed versus normal fibroblasts. Two classes of revertants were isolated: class I revertants have sustained mutations in cellular genes, and a class II revertant has a nonfunctional v-fos provirus. Somatic-cell hybridization studies suggested that the revertant phenotype was recessive to the transformed phenotype. Class I revertants were also resistant to retransformation by v-gag-fos-fox, v-Ha-ras, v-abl, and v-mos, but could be retransformed by the trk oncogene and polyoma virus middle T antigen. These results suggest that the class I revertants sustained mutations in one or more cellular genes essential for transformation by some, but not all, oncogenes. Our data suggest the existence of common biochemical pathways for transformation.
利用一种基于罗丹明123在v-fos转化的成纤维细胞与正常成纤维细胞线粒体中长时间保留的新型筛选程序，分离出了FBJ小鼠肉瘤病毒（v-fos）转化的大鼠-1成纤维细胞的形态学回复突变体。分离出了两类回复突变体：I类回复突变体在细胞基因中存在持续突变，II类回复突变体具有无功能的v-fos原病毒。体细胞杂交研究表明，回复突变体表型相对于转化表型是隐性的。I类回复突变体对v-gag-fos-fox、v-Ha-ras、v-abl和v-mos的再转化也具有抗性，但可被trk癌基因和多瘤病毒中T抗原再转化。这些结果表明，I类回复突变体在一个或多个对某些（但不是所有）癌基因转化至关重要的细胞基因中存在持续突变。我们的数据表明存在共同的转化生化途径。
