利用实时聚合酶链式反应（PCR）建立DNA标记，以区分常被混淆用作草药的[具体物种1]和[具体物种2]。
Establishing DNA markers to differentiate and , which are confusedly used as medicinal herbs, using real-time PCR.
作者信息
Uh Yo Ram, Jang Cheol Seong
机构信息
Plant Genomics Laboratory, Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon, 24341 Republic of Korea.
出版信息
Food Sci Biotechnol. 2022 Oct 1;32(2):239-247. doi: 10.1007/s10068-022-01176-y. eCollection 2023 Feb.
UNLABELLED
and are used as medicinal herbs and aromatic plants and belong to the family . Despite differences in composition and physicochemical properties, both plants are frequently sold as the medical substance "Kwakhyang" in some Asian countries. Molecular markers were established to distinguish between the two plants using quantitative real-time PCR. Species-specific primers were designed in the nuclear internal transcribed spacer region of ribosomal DNA and in the chloroplast genes , , and . Six primer sets were tested, the correlation coefficient was higher than 0.99, and the slope was approximately - 3.36 to - 3.58. Efficiency ranged from 90.13 to 98.52%. The developed real-time PCR assay was validated with 14 off-target species, and its reliability was verified through blind testing of 14 commercial products. The assay developed here may help protect consumer rights, and the designed primers can be used to distinguish between the target species.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-022-01176-y.
未标注
[植物名称1]和[植物名称2]被用作草药和芳香植物，属于[植物科名]科。尽管在成分和理化性质上存在差异，但在一些亚洲国家，这两种植物经常作为药用物质“桂皮”出售。利用定量实时聚合酶链反应（qRT-PCR）建立了区分这两种植物的分子标记。在核糖体DNA的核内转录间隔区以及叶绿体基因[基因名称1]、[基因名称2]和[基因名称3]中设计了物种特异性引物。测试了六组引物，相关系数高于0.99，斜率约为-3.36至-3.58。效率范围为90.13%至98.52%。所开发的实时PCR检测方法用14种非目标物种进行了验证，并通过对14种商业产品的盲测验证了其可靠性。这里开发的检测方法可能有助于保护消费者权益，所设计的引物可用于区分目标物种。
补充信息
在线版本包含可在10.1007/s10068-022-01176-y获取的补充材料。
Zotero 插件