Yoo Seung Hyun, Kim Jeong Gyu, Park Yong Jun, Kim Jong-Oh, Seo Yong Bae, Kim Gun-Do
Department of Microbiology, College of Natural Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513 Republic of Korea.
Research Institute for Basic Sciences, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513 Republic of Korea.
Food Sci Biotechnol. 2025 Apr 10;34(11):2657-2666. doi: 10.1007/s10068-025-01877-0. eCollection 2025 Jul.
Illegal distribution and mislabeling of imported fishery products pose challenges to country-of-origin labeling regulations in Korea. To address this issue, a quantitative PCR (qPCR)-based method was developed to distinguish from Korea and Japan. Using genotyping-by-sequencing (GBS), two single nucleotide polymorphism (SNP) markers were identified, and allele-specific primers were designed. Gaussian mixture modeling established Ct thresholds, achieving accuracy of 81.67% and 77.78% for SNP001 and SNP008, respectively. The amplification efficiency and limit of detection (LOD) were assessed using tenfold serial dilutions (10-0.001 ng/μL). Standard curves for AA and TT homotypes showed high linearity (R > 0.994) with amplification efficiencies of 103.65% and 97.63%, respectively. This qPCR-based method provides a reliable approach for origin verification of , aiding regulatory enforcement and ensuring seafood authenticity.
The online version contains supplementary material available at 10.1007/s10068-025-01877-0.
进口水产品的非法分销和错误标签给韩国的原产国标签规定带来了挑战。为解决这一问题，开发了一种基于定量聚合酶链反应（qPCR）的方法来区分韩国和日本的[具体产品名称未给出]。使用测序基因分型（GBS），鉴定了两个单核苷酸多态性（SNP）标记，并设计了等位基因特异性引物。高斯混合模型确定了Ct阈值，SNP001和SNP008的准确率分别达到81.67%和77.78%。使用十倍系列稀释（10 - 0.001 ng/μL）评估扩增效率和检测限（LOD）。AA和TT同型的标准曲线显示出高线性（R＞0.994），扩增效率分别为103.65%和97.63%。这种基于qPCR的方法为[具体产品名称未给出]的产地验证提供了一种可靠的方法，有助于监管执法并确保海鲜的真实性。
在线版本包含可在10.1007/s10068 - 025 - 01877 - 0获取的补充材料。
