Zhao Yuanyuan, Su Xiaohong
Sexually Transmitted Disease Clinic, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
Biochem Biophys Rep. 2023 Jan 12;33:101427. doi: 10.1016/j.bbrep.2023.101427. eCollection 2023 Mar.
Gonorrhea is the second most common sexually transmitted diseases worldwide. Chronic infection of () can lead to severe complications. Presently, has developed resistance to almost all antibiotics used for the treatment of gonorrhea. Thus, it's urgent to explore new approaches to treat gonorrhea. Presently, nontraditional treatment method as an alternative to antibiotic use is getting more and more attention. Here we demonstrated that 18β-glycyrrhetinic acid (GRA) exhibited robust antimicrobial activity against in vitro. GRA led to a significant decline in viable in a dose dependent manner compared with DMSO treatment (P < 0.001). Addition of GRA resulted in a significant reduction in viable bacteria within 2 h post-inoculation (P < 0.001). Minimum inhibitory concentrations (MICs) to GRA ranged from 3.9 to 62.5 μg/ml overall, with MIC50 and MIC90 values of 31.25 μg/ml and 62.5 μg/ml, respectively. There was no significant difference of MIC 50 and MIC90 between multi-drug resistant (MDR) strains and non-MDR strains. Minimum bactericidal concentration (MBC) ranges were 3.9-125 μg/ml, basically consistent with MIC values. GRA inhibited biofilm formation and diminished pre-formed biofilm. These data suggested that GRA could be a candidate for gonorrhea treatment.
淋病是全球第二常见的性传播疾病。（）的慢性感染可导致严重并发症。目前，（）已对几乎所有用于治疗淋病的抗生素产生耐药性。因此，探索治疗淋病的新方法迫在眉睫。目前，作为抗生素使用替代方法的非传统治疗方法越来越受到关注。在此我们证明，18β-甘草次酸（GRA）在体外对（）表现出强大的抗菌活性。与二甲基亚砜处理相比，GRA以剂量依赖性方式导致活菌数显著下降（P < 0.001）。接种后2小时内添加GRA导致活菌数显著减少（P < 0.001）。总体而言，GRA的最低抑菌浓度（MIC）范围为3.9至62.5μg/ml，MIC50和MIC90值分别为31.25μg/ml和62.5μg/ml。多重耐药（MDR）菌株和非MDR菌株之间的MIC50和MIC90没有显著差异。最低杀菌浓度（MBC）范围为3.9 - 125μg/ml，基本与MIC值一致。GRA抑制生物膜形成并减少预先形成的生物膜。这些数据表明GRA可能是治疗淋病的候选药物。
