Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity (LR11ES39), Team: Medicinal Chemistry and Natural Products, Faculty of Science of Monastir, University of Monastir, Avenue of Environment, 5019, Monastir, Tunisia.
University of Sousse, Regional Center of Research on Horticulture and Organic Agriculture, 57, ChottMariem, TN-4042, Sousse, Tunisia.
Chem Biodivers. 2023 Feb;20(2):e202200646. doi: 10.1002/cbdv.202200646. Epub 2023 Jan 19.
Due to the several side effects of synthetic pesticides, including environmental pollution, threats to human health, and the development of pest resistance to insecticides, the use of alternative healthy, available and efficient agents in pest management strategies is necessary. Recently, the use of essential oil obtained from aromatic plants has shown significant potential for insect pest management. For this reason, the essential oil isolated from seeds of Thapsia garganica L. was investigated for the first time for its chemical profile, and its toxicity and repellency effects against Tribolium castaneum adults. Qualitative and quantitative analyses of the chemical composition by gas chromatography coupled to mass spectrometry (GC/MS) revealed the presence of 18 organic volatiles representing 96.8 % of the total constituents. The main compounds were 1,4-dimethylazulene (51.3 %) followed by methyl palmitate (8.2 %), methyl linoleate (6.2 %) and costol (5.1 %). Concerning the repellent effect, results revealed that SEO (Seed Essential Oil) was very repellent towards T. castaneum adults, with 100 % repellency after 2 h of exposure. Furthermore, the essential oil exhibited remarkable contact toxicity against T. castaneum (93.3 % of mortality) at the concentration of 10 % (v/v). The median lethal dose (LD ) of the topical application of the seed essential oil was 4.4 %. These encouraging outcomes suggested that the essential oil from T. garganica seeds could be considered a potent natural alternative to residual persistent and toxic insecticides.
由于合成农药存在多种副作用，包括环境污染、对人类健康的威胁以及害虫对杀虫剂产生抗药性，因此在害虫管理策略中需要使用其他健康、可用且高效的药剂。最近，从芳香植物中提取的精油在害虫管理方面显示出了巨大的潜力。出于这个原因，首次研究了从 Thapsia garganica L.种子中提取的精油的化学成分、对赤拟谷盗成虫的毒性和驱避作用。通过气相色谱-质谱联用仪（GC/MS）进行的定性和定量化学组成分析表明，存在 18 种有机挥发物，占总成分的 96.8%。主要化合物为 1,4-二甲基薁（51.3%），其次是棕榈酸甲酯（8.2%）、亚油酸甲酯（6.2%）和柯斯塔醇（5.1%）。关于驱避效果，结果表明 SEO（种子精油）对 T. castaneum 成虫非常驱避，暴露 2 小时后驱避率达到 100%。此外，该精油对 T. castaneum 具有显著的接触毒性（死亡率为 93.3%），在 10%（v/v）的浓度下。种子精油的经皮应用的半数致死剂量（LD ）为 4.4%。这些令人鼓舞的结果表明，T. garganica 种子精油可以被认为是一种有潜力的天然替代残留持久性和有毒杀虫剂的选择。
