Li Mengyu, Li Xin, Liu Chuang, Han Jinyi, Han Xuelei, Wang Kejun, Qiao Ruimin, Yang Feng, Li Xiu-Ling, Li Xin-Jian
College of Animal Science and Technology, Henan Agricultural University, Henan, Zhengzhou, China.
Anim Genet. 2023 Jun;54(3):403-407. doi: 10.1111/age.13294. Epub 2023 Jan 17.
Enhancing pig reproductive efficiency has the potential to have a significant positive economic impact on the pig business. We collected four reproduction records of 734 Yunong black pigs in this study, including the total number of piglets born (TNB), the number born alive (NBA), the average birth interval of piglets (ABI) and the average birth weight (ABW). A total of 453 Yunong black pigs were genotyped with Porcine 50K SNP BeadChip. Twenty-five SNPs and 35 genomic areas were found to have a substantial impact on the reproductive performance of Yunong black pigs by single-locus GWAS and single-step GWAS (ssGWAS). For the ssGWAS, we found that the two genomic regions (12.67-13.85 and 14.26-15.01 Mb) on Sus scrofa chromosome X were associated with TNB, NBA and ABI. It is worth noting that CNC10110530 and CNC100141254 significantly affected the TNB by both GWAS methods. Finally, we further determined the gene functions by enrichment analysis and a literature search, and identified 28 of them as candidate genes affecting the reproductive performance of Yunong black pigs, including RET, EIF1AX, NELL2, CTPS2, S100G, RBBP7 and PDHA1. This study further promotes understanding of the genetic mechanism of porcine reproductive performance, and also provides more molecular markers for pig breeding.
提高猪的繁殖效率有可能对养猪业产生重大的积极经济影响。在本研究中，我们收集了734头云农黑猪的四项繁殖记录，包括总产仔数（TNB）、产活仔数（NBA）、仔猪平均出生间隔（ABI）和平均出生体重（ABW）。共有453头云农黑猪用猪50K SNP芯片进行了基因分型。通过单基因座全基因组关联研究（GWAS）和单步GWAS（ssGWAS），发现25个单核苷酸多态性（SNP）和35个基因组区域对云农黑猪的繁殖性能有重大影响。对于ssGWAS，我们发现猪X染色体上的两个基因组区域（12.67 - 13.85和14.26 - 15.01兆碱基）与TNB、NBA和ABI相关。值得注意的是，CNC10110530和CNC100141254在两种GWAS方法中均对TNB有显著影响。最后，我们通过富集分析和文献检索进一步确定了基因功能，并鉴定出其中28个为影响云农黑猪繁殖性能的候选基因，包括RET、EIF1AX、NELL2、CTPS2、S100G、RBBP7和PDHA1。本研究进一步促进了对猪繁殖性能遗传机制的理解，也为猪的育种提供了更多分子标记。
