Chen Jianmei, Wu Ziyi, Chen Ruxue, Huang Zhihui, Han Xuelei, Qiao Ruimin, Wang Kejun, Yang Feng, Li Xin-Jian, Li Xiu-Ling
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Animals (Basel). 2022 Jun 19;12(12):1584. doi: 10.3390/ani12121584.
The reproductive traits of sows are one of the important economic traits in pig production, and their performance directly affects the economic benefits of the entire pig industry. In this study, a total of 895 French Large White pigs were genotyped by GeneSeek Porcine 50K SNP Beadchip and four phenotypic traits of 1407 pigs were recorded, including total number born (TNB), number born alive (NBA), number healthy piglets (NHP) and litter weight born alive (LWB). To identify genomic regions and genes for these traits, we used two approaches: a single-locus genome-wide association study (GWAS) and a single-step GWAS (ssGWAS). Overall, a total of five SNPs and 36 genomic regions were identified by single-locus GWAS and ssGWAS, respectively. Notably, fourof all five significant SNPs were located in 10.72-11.06 Mb on chromosome 7, were also identified by ssGWAS. These regions explained the highest or second highest genetic variance in the TNB, NBA and NHP traits and harbor the protein coding gene . In addition, several candidate genes associated with litter traits were identified, including , , and . Overall, these novel results reflect the polygenic genetic architecture of the litter traits and provide a theoretical reference for the following implementation of molecular breeding.
母猪的繁殖性状是生猪生产中的重要经济性状之一，其表现直接影响整个养猪业的经济效益。在本研究中，使用GeneSeek猪50K SNP芯片对895头法国大白猪进行基因分型，并记录了1407头猪的四个表型性状，包括总产仔数（TNB）、产活仔数（NBA）、健康仔猪数（NHP）和产活仔窝重（LWB）。为了鉴定这些性状的基因组区域和基因，我们采用了两种方法：单基因座全基因组关联研究（GWAS）和单步GWAS（ssGWAS）。总体而言，单基因座GWAS和ssGWAS分别鉴定出了5个单核苷酸多态性（SNP）和36个基因组区域。值得注意的是，所有5个显著SNP中的4个位于7号染色体上10.72 - 11.06兆碱基（Mb）处，ssGWAS也鉴定出了这些区域。这些区域解释了TNB、NBA和NHP性状中最高或第二高的遗传方差，并包含蛋白质编码基因。此外，还鉴定出了几个与窝产性状相关的候选基因，包括 、 、 和 。总体而言，这些新结果反映了窝产性状的多基因遗传结构，并为后续分子育种的实施提供了理论参考。
