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一种高度保守的蛋白酶，酰基氨基酸释放酶（AARE），在Physcomitrella 和拟南芥的衰老中起作用。

A deeply conserved protease, acylamino acid-releasing enzyme (AARE), acts in ageing in Physcomitrella and Arabidopsis.

机构信息

Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestrasse 1, 79104, Freiburg, Germany.

Euro-BioImaging Bio-Hub, EMBL Heidelberg, Meyerhofstraße 1, 69117, Heidelberg, Germany.

出版信息

Commun Biol. 2023 Jan 17;6(1):61. doi: 10.1038/s42003-023-04428-7.

DOI:10.1038/s42003-023-04428-7
PMID:36650210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9845386/
Abstract

Reactive oxygen species (ROS) are constant by-products of aerobic life. In excess, ROS lead to cytotoxic protein aggregates, which are a hallmark of ageing in animals and linked to age-related pathologies in humans. Acylamino acid-releasing enzymes (AARE) are bifunctional serine proteases, acting on oxidized proteins. AARE are found in all domains of life, albeit under different names, such as acylpeptide hydrolase (APEH/ACPH), acylaminoacyl peptidase (AAP), or oxidized protein hydrolase (OPH). In humans, AARE malfunction is associated with age-related pathologies, while their function in plants is less clear. Here, we provide a detailed analysis of AARE genes in the plant lineage and an in-depth analysis of AARE localization and function in the moss Physcomitrella and the angiosperm Arabidopsis. AARE loss-of-function mutants have not been described for any organism so far. We generated and analysed such mutants and describe a connection between AARE function, aggregation of oxidized proteins and plant ageing, including accelerated developmental progression and reduced life span. Our findings complement similar findings in animals and humans, and suggest a unified concept of ageing may exist in different life forms.

摘要

活性氧（ROS）是需氧生命的恒定副产物。过量的 ROS 会导致细胞毒性蛋白质聚集体，这是动物衰老的标志，并与人类的与年龄相关的病理有关。酰基氨基酸释放酶（AARE）是一种双功能丝氨酸蛋白酶，作用于氧化蛋白。AARE 存在于所有生命领域，尽管名称不同，如酰基肽水解酶（APEH/ACPH）、酰基氨基酸肽酶（AAP）或氧化蛋白水解酶（OPH）。在人类中，AARE 功能障碍与与年龄相关的病理有关，而其在植物中的功能则不太清楚。在这里，我们对植物谱系中的 AARE 基因进行了详细分析，并对苔藓Physcomitrella 和被子植物拟南芥中的 AARE 定位和功能进行了深入分析。到目前为止，还没有任何生物体描述过 AARE 功能丧失突变体。我们生成并分析了这些突变体，并描述了 AARE 功能、氧化蛋白聚集与植物衰老之间的联系，包括加速发育进程和缩短寿命。我们的发现补充了动物和人类的类似发现，并表明不同生命形式可能存在统一的衰老概念。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/6c05d6b3d94f/42003_2023_4428_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/b8b1d2586790/42003_2023_4428_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/4c9de5e72ebd/42003_2023_4428_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/5fd4c8c372fd/42003_2023_4428_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/3efbdbb4414b/42003_2023_4428_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/bb75a650dd5a/42003_2023_4428_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/6c05d6b3d94f/42003_2023_4428_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/b8b1d2586790/42003_2023_4428_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/4c9de5e72ebd/42003_2023_4428_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/5fd4c8c372fd/42003_2023_4428_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/3efbdbb4414b/42003_2023_4428_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/bb75a650dd5a/42003_2023_4428_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/45a0/9845386/6c05d6b3d94f/42003_2023_4428_Fig6_HTML.jpg

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