Smith D A, Leung F Y, Jablonsky G, Henderson A R
Department of Clinical Biochemistry, University Hospital (University of Western Ontario), London, Canada.
Clin Chem. 1987 Oct;33(10):1863-8.
In this radioimmunoassay of lactate dehydrogenase-1 (LD-1; EC 1.1.1.27) in human serum we use a commercial LD-1-selective assay system and a goat antiserum. We have determined the fractional rate of disappearance from serum and the half-life of LD-1, in terms of both enzyme activity and enzyme mass, in 21 myocardial infarction patients. Our evidence suggests that this isoenzyme is inactivated in serum. Furthermore, our data suggest that the conventionally accepted half-life of about 110 h for serum LD-1 activity may grossly overestimate the actual LD-1 half-life in many post-myocardial infarction patients.
在这项针对人血清中乳酸脱氢酶 -1（LD -1；EC 1.1.1.27）的放射免疫分析中，我们使用了一种市售的LD -1选择性分析系统和山羊抗血清。我们已经测定了21例心肌梗死患者血清中LD -1活性和酶质量的消失分数率及半衰期。我们的证据表明这种同工酶在血清中被灭活。此外，我们的数据表明，传统上认为血清LD -1活性约110小时的半衰期可能在许多心肌梗死后患者中严重高估了实际的LD -1半衰期。
