You J, Song C, Liang F
School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2022 Dec 20;42(12):1889-1895. doi: 10.12122/j.issn.1673-4254.2022.12.19.
To study the electrophysiological properties of parvalbumin (PV) neurons in the auditory cortex (AC) and its descending auditory projection area in the tail of the striatum (TS).
The stimulation response of PV neuron step current was recorded in PV-Cre-Ai14 mice using in vitro patch clamp technique, and the release characteristics and waveform characteristics of PV neuron action potentials (APs) were analyzed using Clampfit and MATLAB software. The release characteristics of the APs included AP onset, rheobase, average firing rate, F/I slope and spike frequency adaptation (SFA); the waveform characteristics included peak and post potential characteristics.
The PV neurons of the TS and the AC had significantly different electrophysiological characteristics. In terms of peak potential characteristics, the PV neurons in the TS presented with smaller half peak width ( < 0.001) and larger amplitude ( < 0.01) with larger maximum ascending slope ( < 0.01) and maximum descending slope ( < 0.05). For post potential characteristics, the PV neurons in the TS showed a greater post hyperpolarization ( < 0.01) with a shorter time for recovery of the resting potential ( < 0.01). The firing characteristics of the PV neurons of the TS featured a higher AP rheobase ( < 0.01), a larger F/I slope ( < 0.01), a greater firing onset delay ( < 0.001), and a larger SFA ( < 0.01).
The PV neurons in the TS and the AC of mice show significantly different electrophysiological characteristics in processing auditory information.
研究听觉皮层（AC）和纹状体尾部（TS）中降听觉投射区域内小白蛋白（PV）神经元的电生理特性。
采用体外膜片钳技术记录PV-Cre-Ai14小鼠中PV神经元阶跃电流的刺激反应，并使用Clampfit和MATLAB软件分析PV神经元动作电位（AP）的释放特性和波形特性。AP的释放特性包括AP起始、阈强度、平均放电频率、F/I斜率和动作电位频率适应（SFA）；波形特性包括峰电位和后电位特性。
TS和AC的PV神经元具有显著不同的电生理特性。在峰电位特性方面，TS中的PV神经元呈现出较小的半峰宽（<0.001）和较大的幅度（<0.01），最大上升斜率（<0.01）和最大下降斜率较大（<0.05）。对于后电位特性，TS中的PV神经元表现出更大的超极化后电位（<0.01），静息电位恢复时间更短（<0.01）。TS中PV神经元的放电特性表现为更高的AP阈强度（<0.01）、更大的F/I斜率（<0.01）、更大的放电起始延迟（<0.001）和更大的SFA（<0.01）。
小鼠TS和AC中的PV神经元在处理听觉信息时表现出显著不同的电生理特性。
