Foged Mads Møller, Maeda Kenji, Bilgin Mesut
Cell Death and Metabolism Unit, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center, Copenhagen, Denmark.
Lipidomics Core Facility, Center for Autophagy, Recycling and Disease, Danish Cancer Society Research Center , Copenhagen, Denmark.
Methods Mol Biol. 2023;2625:89-102. doi: 10.1007/978-1-0716-2966-6_8.
The emerging field of lipidomics presents the systems biology approach to identify and quantify the full lipid repertoire of cells, tissues, and organisms. The importance of the lipidome is demonstrated by a number of biological studies on dysregulation of lipid metabolism in human diseases such as cancer, diabetes, and neurodegenerative diseases. Exploring changes and regulations in the huge networks of lipids and their metabolic pathways requires a lipidomics methodology: advanced mass spectrometry that resolves the complexity of the lipidome. Here, we report a comprehensive protocol of quantitative shotgun lipidomics that enables identification and quantification of hundreds of molecular lipid species, covering a wide range of lipid classes, extracted from cultured mammalian cells.
脂质组学这一新兴领域采用系统生物学方法来识别和量化细胞、组织及生物体中的全部脂质种类。许多关于人类疾病（如癌症、糖尿病和神经退行性疾病）中脂质代谢失调的生物学研究都证明了脂质组的重要性。探索庞大的脂质网络及其代谢途径中的变化和调控需要一种脂质组学方法：能够解析脂质组复杂性的先进质谱技术。在此，我们报告了一种全面的鸟枪法定量脂质组学方案，该方案能够识别和量化从培养的哺乳动物细胞中提取的数百种分子脂质种类，涵盖广泛的脂质类别。
