Neag Emily J, Collao Vanessa, Bhattacharya Sanjoy K
Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, USA.
Methods Mol Biol. 2023;2625:141-148. doi: 10.1007/978-1-0716-2966-6_13.
An optimized Bligh and Dyer protocol and subsequent derivatization is described in this chapter for the extraction of free cholesterol and cholesterol esters from tissue samples. Quantification analysis of lipid species is then described utilizing gas chromatography-mass spectrometry, the ideal method for analysis of volatile organic compounds and extraction of sterols.
本章介绍了一种优化的布莱和戴尔方法及其后续衍生化过程，用于从组织样本中提取游离胆固醇和胆固醇酯。随后描述了利用气相色谱 - 质谱联用技术对脂质种类进行定量分析，这是分析挥发性有机化合物和提取甾醇的理想方法。
