Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Miami Integrative Metabolomics Research Center, Miami, FL, USA.
Methods Mol Biol. 2023;2571:143-148. doi: 10.1007/978-1-0716-2699-3_14.
Cholesterol is an essential lipid molecule for several biological functions including the proper functioning of cell membranes, lipoproteins, and lipid rafts, as well as the synthesis of bile acids, vitamin D, and steroid hormones. Cholesterol can be extracted from liver tissue by multiple methods of lipid extraction. Subsequently, gas chromatography-mass spectrometry (GC-MS) can be used to obtain the highest level of sensitivity and selectivity in the analysis of cholesterol. This chapter describes two methods of lipid extraction for liver tissue, Bligh and Dyer and methyl tertiary butyl ether (MTBE), followed by an analysis with GC-MS.
胆固醇是一种重要的脂质分子，对于多种生物学功能至关重要，包括细胞膜、脂蛋白和脂质筏的正常功能，以及胆汁酸、维生素 D 和甾体激素的合成。胆固醇可以通过多种脂质提取方法从肝组织中提取。随后，气相色谱-质谱联用（GC-MS）可用于在胆固醇分析中获得最高的灵敏度和选择性。本章描述了两种肝组织脂质提取方法，即 Bligh 和 Dyer 法和甲基叔丁基醚（MTBE）法，然后用 GC-MS 进行分析。
