Gonzalez-Granado Jose M, Del Monte-Monge Alberto, Piqueras Laura, Andres Vicente, Rius Cristina
Department of Immunology, Ophthamology and ENT, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain.
Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.
Methods Mol Biol. 2023;2608:451-467. doi: 10.1007/978-1-0716-2887-4_25.
Monocytes play essential roles in the inflammatory and anti-inflammatory processes that take place during an immune response, acting both within the vascular network and interstitially. Monocytes are activated, mobilized, and recruited in response to an inflammatory stimulus or different forms of tissue injury. The recruitment of circulating monocytes to the inflamed tissue is essential to resolving the injury.Monocyte recruitment is a multistep process that begins with a decrease in rolling velocity, is followed by adhesion to the endothelium and crawling over the luminal vessel surface, and culminates in monocyte transmigration into the surrounding tissue. Intravital microscopy is a powerful visualization tool for the study of leukocyte behavior and function, intercellular interactions, cell trafficking, and recruitment in pathological and physiological conditions. This modality is therefore widely used for the detailed analysis of the immune response to multiple insults and the molecular mechanisms underlying monocyte interactions within the vascular system in vivo. This chapter describes a protocol for the use of intravital microscopy to analyze monocyte recruitment from the blood vessel to the inflammatory site.
单核细胞在免疫反应期间发生的炎症和抗炎过程中发挥着重要作用，在血管网络内和间质中均有作用。单核细胞会因炎症刺激或不同形式的组织损伤而被激活、动员和募集。循环单核细胞募集到炎症组织对于损伤的解决至关重要。单核细胞募集是一个多步骤过程，始于滚动速度降低，接着是黏附于内皮并在管腔血管表面爬行，最终单核细胞迁移到周围组织。活体显微镜检查是研究白细胞行为和功能、细胞间相互作用、细胞运输以及病理和生理条件下募集的强大可视化工具。因此，这种方法被广泛用于详细分析对多种损伤的免疫反应以及体内血管系统内单核细胞相互作用的分子机制。本章描述了一种使用活体显微镜检查来分析单核细胞从血管募集到炎症部位的方案。
