Rius Cristina, Sanz María J
Laboratory of Molecular and Genetic Cardiovascular Pathophysiology, Vascular Biology Program, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, Madrid, Spain.
Department of Pharmacology, Faculty of Medicine, University of Valencia, Avenida de Blasco Ibanez, 17, 46010, Valencia, Spain.
Methods Mol Biol. 2015;1339:357-66. doi: 10.1007/978-1-4939-2929-0_26.
The intravital microscopy in the mouse cremaster muscle microcirculation is a method widely used to visualize in vivo blood cells interacting with the endothelium and within the vessels. Therefore, it is a suitable technique to study leukocyte-endothelial cell interactions along every stage of the canonical leukocyte recruitment cascade: rolling, adhesion, intravascular crawling, and migration both in postcapillary venules and arterioles of the mouse cremasteric microcirculation. This technique also enables to assess vessel functionality, since hemodynamic parameters such as shear stress, flow rate, and vasodilatation/vasoconstriction, among other vascular events, can be additionally determined. Furthermore, response to multiple drugs and mechanisms underlying blood cells interactions within the vascular system can be studied in a real scenario. This chapter describes a protocol for intravital microscopy in the mouse cremaster muscle microcirculation.
小鼠提睾肌微循环的活体显微镜检查是一种广泛用于可视化体内血细胞与内皮细胞相互作用以及血管内情况的方法。因此，它是研究经典白细胞募集级联反应各个阶段白细胞与内皮细胞相互作用的合适技术，这些阶段包括在小鼠提睾肌微循环的毛细血管后微静脉和小动脉中的滚动、黏附、血管内爬行和迁移。该技术还能够评估血管功能，因为可以额外测定诸如剪切应力、流速和血管舒张/血管收缩等血流动力学参数以及其他血管事件。此外，可以在实际场景中研究对多种药物的反应以及血管系统内血细胞相互作用的潜在机制。本章描述了小鼠提睾肌微循环活体显微镜检查的方案。
