Gupta Vaishali, Odaneth Annamma A, Lali Arvind M
DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India.
Institute of Chemical Technology, Mumbai, India.
Prep Biochem Biotechnol. 2023 Oct;53(9):1043-1057. doi: 10.1080/10826068.2023.2166956. Epub 2023 Jan 19.
Commercial production of lactic acid (LA) utilizes mostly glucose or lactose coupled with yeast extract (YE) as a supplement. With sugars, nitrogen, and vitamin supplementation being most of the LA production costs, the use of inexpensive molasses, a by-product of the sugar industry, can provide considerable cost savings. There are just a few publications on the production of LA from molasses; consequently, the present investigation was conducted using molasses supplemented with yeast extract. The research was done in a continuous-flow, high-cell-density (HCD) bioreactor with an external membrane microfiltration device for cell recycling. The system, run at 1 L with NCIM 2025, produced a LA yield of 0.95-0.98 g/g from ∼100 g sugars/L when supplemented with 1 g/L YE. Dilution rates in the range of 0.04-0.36 h resulted in volumetric lactic acid productivities in the range of 4.3-27.6 g/L h, which compares favorably with the highest values recorded in literature, for glucose in the presence of YE, which was as high as 30 g/L. The utilization of cane molasses has a significant impact on the economics of lactic acid production, as measured by a comparison of costs with commercial glucose.
乳酸（LA）的商业化生产大多利用葡萄糖或乳糖，并辅以酵母提取物（YE）作为补充剂。由于糖、氮和维生素的补充占了LA生产成本的大部分，使用廉价的糖蜜（制糖工业的副产品）可以大幅节省成本。关于用糖蜜生产LA的出版物很少；因此，本研究使用添加了酵母提取物的糖蜜进行。该研究在一个连续流、高细胞密度（HCD）生物反应器中进行，该反应器带有用于细胞循环的外部膜微滤装置。该系统使用NCIM 2025，在1升的规模下运行，当添加1克/升的YE时，从约100克糖/升中产生的LA产量为0.95 - 0.98克/克。0.04 - 0.36小时的稀释率导致乳酸体积生产率在4.3 - 27.6克/升·小时的范围内，这与文献中在YE存在下葡萄糖的最高记录值相比具有优势，文献中该值高达30克/升。通过与商业葡萄糖的成本比较可知，甘蔗糖蜜的利用对乳酸生产的经济性有重大影响。
