Taizhou Key Laboratory of Biomass Functional Materials Development and Application, Taizhou University, Taizhou 318000, China.
Taizhou Institute of Product Quality and Safety Inspection, Taizhou 318000, China.
Molecules. 2023 Aug 24;28(17):6234. doi: 10.3390/molecules28176234.
As a biodegradable and renewable material, polylactic acid is considered a major environmentally friendly alternative to petrochemical plastics. Microbial fermentation is the traditional method for lactic acid production, but it is still too expensive to compete with the petrochemical industry. Agro-industrial wastes are generated from the food and agricultural industries and agricultural practices. The utilization of agro-industrial wastes is an important way to reduce costs, save energy and achieve sustainable development. The present study aimed to develop a method for the valorization of waste and cane molasses as carbon sources for L-lactic acid fermentation using LA-UN-1. The results showed that xylose derived from the acid hydrolysis of waste was beneficial for cell growth, while glucose from the acid hydrolysis of waste and mixed sugars (glucose and fructose) from the acid hydrolysis of cane molasses were suitable for the accumulation of lactic acid. Thus, a three-stage carbon source utilization strategy was developed, which markedly improved lactic acid production and productivity, respectively reaching 129.47 g/L and 1.51 g/L·h after 86 h of fermentation. This work demonstrates that inexpensive waste and cane molasses can be suitable carbon sources for lactic acid production, offering an efficient utilization strategy for agro-industrial wastes.
聚乳酸作为一种可生物降解和可再生的材料，被认为是石化塑料的主要环保替代品。微生物发酵是生产乳酸的传统方法，但与石化工业相比，成本仍然过高。农业工业废物是由食品和农业工业以及农业实践产生的。利用农业工业废物是降低成本、节约能源和实现可持续发展的重要途径。本研究旨在开发一种利用农业废弃物和甘蔗糖蜜作为碳源，通过 LA-UN-1 发酵生产 L-乳酸的方法。结果表明，农业废弃物酸水解得到的木糖有利于细胞生长，而农业废弃物酸水解得到的葡萄糖和甘蔗糖蜜酸水解得到的混合糖（葡萄糖和果糖）则适合乳酸的积累。因此，开发了一种三阶段碳源利用策略，分别在 86 小时发酵后达到 129.47 g/L 和 1.51 g/L·h 的乳酸产量和产率。这项工作表明，廉价的农业废弃物和甘蔗糖蜜可以作为生产乳酸的合适碳源，为农业工业废物的高效利用提供了一种策略。
