Ai Mingkun, Han Feng, Yang Xiangyi, Chu Hongyi, Luo Chuan, Tan Shixin, Lv Sainan, Qin Minjian, Xie Guoyong
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China.
J Appl Microbiol. 2023 Feb 16;134(2). doi: 10.1093/jambio/lxad010.
The main purpose of this study was to study the preventive effect of Penicillium sp. CX-1 on Phytophthora cactorum causing Salvia miltiorrhiza blight and its positive effect on plant growth.
The endophytic strain CX-1 was isolated from the medicinal plant Corydalis saxicola Bunting and identified as Penicillium oxalicum. The growth inhibitory capacity of CX-1 against Ph. cactorum was 74.4% in the strain co-culture test and 86.2% in filtrate-modified plates. In the pot experiment, the in vivo control of CX-1 against Ph. cactorum in S. miltiorrhiza was 36.0%, which was higher than that of an anti-Phytophthora fungicide (23.4%). In addition, CX-1 had a potent ability to solubilize phosphate and also showed the ability to produce the plant hormone indole-3-acetic acid (IAA) and siderophores, which increase the bioavailability of iron to plants. It was demonstrated through pot experiments that CX-1 could significantly promote plant growth. As determined by real-time quantitative PCR, the expression of some S. miltiorrhiza tanshinone-related biosynthesis genes was significantly upregulated following colonization by CX-1.
Strain CX-1 could effectively inhibit Ph. cactorum, the causative agent of S. miltiorrhiza blight, and significantly promoted the growth of plants through several different routes.
本研究的主要目的是研究青霉属菌株CX-1对引起丹参疫病的恶疫霉的防治效果及其对植物生长的积极作用。
从药用植物岩黄连中分离出内生菌株CX-1，并鉴定为草酸青霉。在菌株共培养试验中，CX-1对恶疫霉的生长抑制能力为74.4%，在滤液改良平板试验中为86.2%。在盆栽试验中，CX-1对丹参中恶疫霉的体内防治效果为36.0%，高于一种抗疫霉杀菌剂（23.4%）。此外，CX-1具有较强的解磷能力，还表现出产生植物激素吲哚-3-乙酸（IAA）和铁载体的能力，铁载体可提高植物对铁的生物利用率。盆栽试验表明，CX-1能显著促进植物生长。通过实时定量PCR测定，CX-1定殖后，一些丹参丹参酮相关生物合成基因的表达显著上调。
菌株CX-1能有效抑制丹参疫病病原菌恶疫霉，并通过多种不同途径显著促进植物生长。
