Yang Xiangyi, Cheng Xinyi, Zhao Ziyi, Ai Mingkun, Liu Hui, Zhu Yan, Han Feng, Qin Minjian, Xie Guoyong
Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China.
Yangzhou Center for Food and Drug Control, Yangzhou, China.
Microbiol Spectr. 2025 Sep 2;13(9):e0309424. doi: 10.1128/spectrum.03094-24. Epub 2025 Jul 30.
The objective of this study was to evaluate the feasibility of using the endophytic fungus J7 as a biocontrol agent for blight, and to provide ideas for sources and practical applications of biocontrol agents. sp. J7 was isolated from L. and identified as . The growth inhibition capacity of J7 against , the cause of blight in was 90.0% in filtrate-modified plates, and the incidence of blight in potted plants was reduced by 14.8%, surpassing the 7.4% reduction in the positive control group treated with cymoxanil plus mancozeb. Strain J7 also significantly increased the fresh weight and height of potted seedlings by 2.39 and 1.37 times, respectively. Furthermore, within 5 months of inoculation, the tanshinone content in the field-grown increased significantly compared to control plants. Additionally, the mechanisms of growth promotion, antimicrobial activity, and regulation of secondary metabolites by strain J7 were explored, which strongly support the practical application of this fungal preparation in controlling blight.IMPORTANCE can cause various plant diseases, and currently available control methods are still limited. Existing species show certain limitations in the control of plant diseases caused by . This study confirms a promising biological control agent that can be used to control and provides additional information on the biological characteristics of .
本研究的目的是评估使用内生真菌J7作为防治疫病的生物防治剂的可行性，并为生物防治剂的来源和实际应用提供思路。从[植物名称]中分离出菌株J7，并鉴定为[具体菌种]。在滤液改良平板上，J7对引起[植物名称]疫病的[病原菌名称]的生长抑制能力为90.0%，盆栽植物中疫病的发病率降低了14.8%，超过了用霜脲氰加代森锰锌处理的阳性对照组7.4%的降低率。菌株J7还显著增加了盆栽[植物名称]幼苗的鲜重和株高，分别增加了2.39倍和1.37倍。此外，接种后5个月内，田间种植的[植物名称]中丹参酮含量与对照植株相比显著增加。此外，还探索了菌株J7促进生长、抗菌活性和调节次生代谢产物的机制，这有力地支持了这种真菌制剂在防治[植物名称]疫病中的实际应用。重要性[病原菌名称]可引起多种植物病害，目前可用的防治方法仍然有限。现有的[微生物名称]物种在防治由[病原菌名称]引起的植物病害方面存在一定局限性。本研究证实了一种有前途的可用于防治[植物名称]疫病的生物防治剂，并提供了关于[病原菌名称]生物学特性的更多信息。
