CF3的基因组分析揭示了促进植物生长和抗病的潜力。

Genomic Analysis of CF3 Reveals Potential for Plant Growth Promotion and Disease Resistance.

作者信息

Yang Jianfei, Zang Wenshuai, Chen Jie, Lu Dongying, Li Ruotong, Li Ciyun, Chen Yinhua, Liu Qin, Niu Xiaolei

机构信息

School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.

National Key Laboratory for Tropical Crop Breeding, Sanya 572025, China.

出版信息

J Fungi (Basel). 2025 Feb 17;11(2):153. doi: 10.3390/jof11020153.

DOI:10.3390/jof11020153

PMID:39997447

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11856539/

Abstract

CF3 is a fungus isolated from healthy strawberry soil, with the potential to promote the growth of plants and enhance their resistance to diseases. However, the genome sequence of CF3 remains unclear. Therefore, we performed the whole-genome CCS sequencing of CF3 using the PacBio Sequel II platform. The assembled genome comprised 104 contigs, with a total length of 37,564,657 bp, encoding 13,252 protein-coding genes. Comprehensive functional annotation was performed using various BLAST databases, including the non-redundant (Nr) protein sequence database, Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), EuKaryotic Orthologous Groups (KOG), and the Carbohydrate-Active enZymes (CAZy) database, to identify and predict protein-coding genes, tRNAs, and rRNAs. The Antibiotics and Secondary Metabolites Analysis Shell (Antismash) analysis identified 50 biosynthetic gene clusters involved in secondary metabolite production within the CF3 genome. The whole-genome sequencing of CF3 helps us to understand its potential mechanisms in promoting plant growth and enhancing disease resistance, paving the way for the application of the CF3 strain in sustainable crop production.

摘要

CF3是一种从健康草莓土壤中分离出的真菌，具有促进植物生长和增强植物抗病能力的潜力。然而，CF3的基因组序列仍不清楚。因此，我们使用PacBio Sequel II平台对CF3进行了全基因组CCS测序。组装后的基因组由104个重叠群组成，总长度为37,564,657 bp，编码13,252个蛋白质编码基因。使用各种BLAST数据库进行了全面的功能注释，包括非冗余（Nr）蛋白质序列数据库、基因本体论（GO）、京都基因与基因组百科全书（KEGG）、真核直系同源组（KOG）和碳水化合物活性酶（CAZy）数据库，以识别和预测蛋白质编码基因、tRNA和rRNA。抗生素和次生代谢产物分析外壳（Antismash）分析在CF3基因组中鉴定出50个参与次生代谢产物产生的生物合成基因簇。CF3的全基因组测序有助于我们了解其促进植物生长和增强抗病性的潜在机制，为CF3菌株在可持续作物生产中的应用铺平了道路。