Parihar Akrity, Malaviya Piyush
Department of Environmental Sciences, University of Jammu, Jammu, 180006, J&K, India.
Department of Environmental Sciences, University of Jammu, Jammu, 180006, J&K, India.
Environ Res. 2023 Mar 15;221:115307. doi: 10.1016/j.envres.2023.115307. Epub 2023 Jan 17.
The study aims at developing a phyto-microremediation system for textile wastewater treatment using Spirodela polyrhiza (L.) Schleid. and a consortium of bacterial strains isolated from textile wastewater-contaminated matrices and rhizosphere of S. polyrhiza. The sequential phyto-microremediation of textile wastewater was carried out utilizing two-stage phyto-microremediation systems I [phytoremediation system (Stage 1) preceded microremediation system (Stage 2)] and II [microremediation system (Stage 1) preceded phytoremediation system (Stage 2)]. Pseudomonas stutzeri, Janibacter anophelis, Bacillus safensis, Bacillus pumilus, Bacillus thuringiensis, and Bacillus cereus constituted the bacterial consortium that was involved in the microremediation of textile wastewater. Biochemical characterization of Spirodela on exposure to untreated textile wastewater showed cadmium and nickel uptake as 26.03 and 22.99 mg g dw. S. polyrhiza exhibited anatomical changes like distortion in the structure of the xylem, phloem, lower epidermis, and increased aerenchyma formation when remediating textile wastewater. The textile wastewater bioremediation in phyto-microremediation system I gives final reduction of COD 77.36%, color 91.70%, calcium 61.65%, iron 69.41%, nickel 89.30%, cadmium 88.37%, nitrate 70.83%, phosphate 73.11%, and sulfate 75.49%. Further, LC-MS analysis of treated wastewater from phyto-microremediation system I have shown biotransformation of metabolites into simpler compounds like 2-{Bis [4-(2-cyanophenoxy)phenyl]methyl}benzoic acid (CHNO). The FTIR spectrum of bacterial biomass exposed to textile wastewater exhibits substantial shifts of various bands in the IR region for functional groups such as alcohol, alkene, esters, azide, and amine as compared to non-exposed biomass.
本研究旨在开发一种利用紫萍（Spirodela polyrhiza (L.) Schleid.）以及从受纺织废水污染的基质和紫萍根际分离出的一组细菌菌株处理纺织废水的植物-微生物修复系统。利用两级植物-微生物修复系统I[植物修复系统（第1阶段）先于微生物修复系统（第2阶段）]和II[微生物修复系统（第1阶段）先于植物修复系统（第2阶段）]对纺织废水进行顺序植物-微生物修复。施氏假单胞菌、嗜蚊詹氏菌、安全芽孢杆菌、短小芽孢杆菌、苏云金芽孢杆菌和蜡样芽孢杆菌构成了参与纺织废水微生物修复的细菌群落。紫萍暴露于未处理的纺织废水后的生化特性表明，其对镉和镍的吸收量分别为26.03和22.99 mg/g干重。紫萍在修复纺织废水时表现出解剖学变化，如木质部、韧皮部、下表皮结构扭曲以及通气组织形成增加。植物-微生物修复系统I对纺织废水的生物修复最终使化学需氧量（COD）降低77.36%、颜色降低9l.70%、钙降低61.65%、铁降低69.41%、镍降低89.30%、镉降低88.37%、硝酸盐降低70.83%、磷酸盐降低73.11%、硫酸盐降低75.49%。此外，对植物-微生物修复系统I处理后的废水进行液相色谱-质谱联用（LC-MS）分析表明，代谢产物已生物转化为更简单的化合物，如2-{双[4-(2-氰基苯氧基)苯基]甲基}苯甲酸（CHNO）。与未暴露于纺织废水的细菌生物质相比，暴露于纺织废水的细菌生物质的傅里叶变换红外光谱（FTIR）显示，红外区域中醇、烯烃、酯、叠氮化物和胺等官能团的各种谱带发生了显著位移。
