Li Junhui, Wang Lu, Yang Kun, Zhang Guocai, Li Shan, Gong Hongjian, Liu Mingqi, Dai Xianjun
College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China; Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University, Linyi, China.
College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang 310018, China.
Carbohydr Polym. 2023 Mar 1;303:120467. doi: 10.1016/j.carbpol.2022.120467. Epub 2022 Dec 16.
Pectic polysaccharide has attracted increasing attention for their potential biological properties and applications in health industries. In this study, a low-molecular-weight pectic polysaccharide, POS4, was obtained from citrus peel. The structure of POS4 was preliminarily analyzed by gel-permeation chromatography, monosaccharide analysis, infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR). Results showed that the molecular weight of POS4 was 4.76 kDa and it was a galacturonic acid enriched pectic polysaccharide. The anti-aging activity in vivo showed that POS4 could notably prolong the average lifespan of fruit flies by suppressing the generation of reactive oxygen species (ROS). Further studies demonstrated that POS4 could enhance intestinal homeostasis by modulating gut microbiota in a positive way and regulating autophagy associated genes. Taken together, we proposed that galacturonic acid enriched low molecular weight pectic polysaccharide have great potential in the development of healthy foods such as anti-aging health care products.
果胶多糖因其潜在的生物学特性及其在健康产业中的应用而受到越来越多的关注。在本研究中，从柑橘皮中获得了一种低分子量果胶多糖POS4。通过凝胶渗透色谱、单糖分析、红外光谱（IR）和核磁共振光谱（NMR）对POS4的结构进行了初步分析。结果表明，POS4的分子量为4.76 kDa，是一种富含半乳糖醛酸的果胶多糖。体内抗衰老活性表明，POS4可通过抑制活性氧（ROS）的产生显著延长果蝇的平均寿命。进一步的研究表明，POS4可以通过正向调节肠道微生物群和调节自噬相关基因来增强肠道稳态。综上所述，我们认为富含半乳糖醛酸的低分子量果胶多糖在抗衰老保健品等健康食品的开发中具有巨大潜力。
