School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, PR China; Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing, 100049, PR China.
Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing, 100049, PR China.
Carbohydr Polym. 2018 Apr 15;186:168-175. doi: 10.1016/j.carbpol.2018.01.026. Epub 2018 Jan 11.
Many studies suggested that polysaccharides could impact on the gut microbiota. To discover new polysaccharides which influence intestinal beneficial bacteria, a pectin polysaccharide FMP-6-S2 with an average molecular weight of 86.83 kDa was purified from Fructus Mori. The monosaccharide residue analysis indicated that FMP-6-S2 was composed of rhamnose, galacturonic acid, galactose and arabinose in a molar ratio of 30.86: 24.78: 28.70: 15.61. The backbone of FMP-6-S2 contained 1, 4-linked α-GalpA and 1, 2-linked α-Rhap with branches substituted at C-4 position of rhamnose. The branches were composed of 1, 4-linked β-Galp, terminal (T) - and 1, 3, 6-linked β-Galp, T- and 1, 5-linked α-Araf. Bioactivity test results suggested that FMP-6-S2 and its degraded product could promote growth of intestinal bacteria, B. thetaiotaomicron, which is a dominate strain in the gut of human to benefit intestinal mucosa. These results suggested that FMP-6-S2 and its degraded product might improve human wellness by modulating B. thetaiotaomicron.
许多研究表明，多糖可以影响肠道微生物群。为了发现影响肠道有益细菌的新多糖，我们从桑椹中提取了一种平均分子量为 86.83 kDa 的果胶多糖 FMP-6-S2。单糖残基分析表明，FMP-6-S2 由鼠李糖、半乳糖醛酸、半乳糖和阿拉伯糖以摩尔比 30.86:24.78:28.70:15.61 组成。FMP-6-S2 的主链含有 1,4-连接的α-GalpA 和 1,2-连接的α-Rhap，在鼠李糖的 C-4 位有支链取代。支链由 1,4-连接的β-Galp、末端（T）-和 1,3,6-连接的β-Galp、T-和 1,5-连接的α-Araf 组成。活性测试结果表明，FMP-6-S2 及其降解产物可以促进肠道细菌 B. thetaiotaomicron 的生长，B. thetaiotaomicron 是人类肠道中占优势的菌株，有益于肠道黏膜。这些结果表明，FMP-6-S2 及其降解产物可能通过调节 B. thetaiotaomicron 来改善人类健康。
