Rezvan Hossein, Ali Selman A, Hamoon Navard Sahar
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
Interdisciplinary Biomedical Research Centre, School of Science and Technology, Nottingham Trent University, Nottingham, UK.
Iran J Parasitol. 2022 Oct-Dec;17(4):562-572. doi: 10.18502/ijpa.v17i4.11284.
is a parasite causing leishmaniasis with different clinical manifestations depending on the infectious species in many countries worldwide. Although different studies have been taken place to clear the interaction of the parasite with the immune system, many aspects of immunology of leishmaniasis is remained uncertain.
Bone marrow derived dendritic cells (DCs) were cultured in vitro and divided into different groups (Nottingham Trent University, Nottingham, UK). The groups were separately infected with live or autoclaved or loaded with Soluble Antigen (SLA). The expression of major histocompatibility complex class I (MHC-I) molecule was checked and compared on the cultured DCs using flow cytometry.
Infection of caused a significant downregulation in expression of molecules where killed or SLA could not induce suppression in expression of these molecules.
results in downregulation of MHC-I expression on bone marrow-derived dendritic cells.
是一种导致利什曼病的寄生虫，在世界许多国家，其临床表现因感染种类而异。尽管已经进行了不同的研究来阐明该寄生虫与免疫系统的相互作用，但利什曼病免疫学的许多方面仍不确定。
体外培养骨髓来源的树突状细胞（DCs）并分为不同组（英国诺丁汉诺丁汉特伦特大学）。这些组分别用活的、高压灭菌的或加载可溶性抗原（SLA）的进行感染。使用流式细胞术检查并比较培养的DCs上主要组织相容性复合体I类（MHC-I）分子的表达。
感染导致分子表达显著下调，而灭活的或SLA不能诱导这些分子表达的抑制。
导致骨髓来源的树突状细胞上MHC-I表达下调。
