Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, Thailand.
Chulalongkorn University Demonstration Secondary School, Faculty of Education, Chulalongkorn University, Pathum Wan, Bangkok, Thailand.
PLoS One. 2023 Jan 20;18(1):e0280673. doi: 10.1371/journal.pone.0280673. eCollection 2023.
Culcioides biting midges (Diptera: Ceratopogonidae) are vectors of various veterinary pathogens. Suction light traps are one of the most widely used tools for vector surveillance. The present aim was to compare the efficiency for the collection of Culicoides species between newly developed 3D-printed ultraviolet (Mahidol University (MU) UV LED) and green light-emitting diode (Mahidol University (MU) Green LED) traps baited with CO2 and UV LED Center for Disease Control (CDC) light trap (BioQuip 2770) baited with CO2. The experiment consisted of two replicates of a 3 × 3 Latin square design in each three sampling locations (Location 1, 2, 3 and 4, 5, 6), for 12 nights between 26th July and 7th August 2020 in Thailand. Results showed that efficiency of the MU UV LED light trap was equivalent to that of the BioQuip 2770 trap for the collection of Culicoides. Meanwhile, the efficiency of the MU Green LED light trap was lower than that of both UV LED light traps. In the analysis of Culicoides species composition and sex-age grading, a similar pattern was observed among three light traps except for Culicoides actoni Smith. The newly developed 3D-printed UV LED light trap demonstrated the following advantages over the commercial light trap: cost saving to obtain multiple units, ease of customization and standardization, and increased availability by end-users. Although further assessments in different environmental conditions are needed, this 3D-printed light trap design could minimize the constrains in vector surveillance programs worldwide.
吸血蠓（双翅目：蠓科）是多种兽医病原体的传播媒介。吸虫灯是用于媒介监测的最广泛使用的工具之一。本研究旨在比较新开发的 3D 打印紫外（玛希隆大学（MU）UV LED）和绿色发光二极管（玛希隆大学（MU）Green LED）诱虫器与 CO2 和紫外 LED 疾病控制中心（CDC）诱虫器（BioQuip 2770）在收集 Culicoides 物种方面的效率，后者用 CO2 诱饵。该实验由在泰国 2020 年 7 月 26 日至 8 月 7 日期间的三个采样地点（地点 1、2、3 和 4、5、6）的每个三个拉丁方设计的两个重复组成，共 12 晚。结果表明，MU UV LED 诱虫器的效率与 BioQuip 2770 诱虫器相当，可用于收集吸血蠓。同时，MU Green LED 诱虫器的效率低于两种紫外 LED 诱虫器。在吸血蠓物种组成和性别年龄分级分析中，三种诱虫器之间观察到类似的模式，除了 Culicoides actoni Smith 之外。新开发的 3D 打印紫外 LED 诱虫器与商用诱虫器相比具有以下优势：获得多个单位可节省成本，易于定制和标准化，并且最终用户的可用性增加。尽管需要在不同的环境条件下进行进一步评估，但这种 3D 打印诱虫器设计可以最大限度地减少全球媒介监测计划中的限制。
