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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Guarnieri C, Flamigni F, Rizzuto S, Vaona I, Caldarera C M

Istituto di Chimica Biologica, Università di Bologna, Italy.

Int J Biochem. 1987;19(10):931-5. doi: 10.1016/0020-711x(87)90174-1.

The perfusion for 15 min of isolated rat hearts with 100 microM t-butylhydroperoxide leads to a 75% diminuition of the tissue GSH/GSSG ratio. 2. After t-butylhydroperoxide infusion, the isoproterenol-stimulated heart ODC was strongly inhibited. The addition of 2 mM DTT in the assaying buffer removed the ODC inactivation. 3. The inhibited ODC had an eluition profile similar to active ODC when chromatographed on a Sephacryl S-200 column; moreover, the ODC activity recovered after a thiol affinity chromatography as unbound fraction, was two times increased in the t-butylhydroperoxide perfused hearts in comparison to control. 4. The hearts perfused with 1 mM acetylcysteine after 15 min of perfusion with t-butylhydroperoxide recovered almost completely the initial ODC activity.

用100微摩尔叔丁基过氧化氢对离体大鼠心脏灌注15分钟，会导致组织谷胱甘肽/氧化型谷胱甘肽比值降低75%。2. 输注叔丁基过氧化氢后，异丙肾上腺素刺激的心脏鸟氨酸脱羧酶（ODC）受到强烈抑制。在测定缓冲液中加入2毫摩尔二硫苏糖醇（DTT）可消除ODC的失活。3. 当在Sephacryl S - 200柱上进行色谱分析时，受抑制的ODC具有与活性ODC相似的洗脱图谱；此外，经硫醇亲和色谱作为未结合部分回收后，叔丁基过氧化氢灌注心脏中的ODC活性与对照相比增加了两倍。4. 在叔丁基过氧化氢灌注15分钟后，用1毫摩尔乙酰半胱氨酸灌注的心脏几乎完全恢复了初始ODC活性。

Guarnieri C, Flamigni F, Rizzuto S, Vaona I, Caldarera C M

Istituto di Chimica Biologica, Università di Bologna, Italy.

Int J Biochem. 1987;19(10):931-5. doi: 10.1016/0020-711x(87)90174-1.

The perfusion for 15 min of isolated rat hearts with 100 microM t-butylhydroperoxide leads to a 75% diminuition of the tissue GSH/GSSG ratio. 2. After t-butylhydroperoxide infusion, the isoproterenol-stimulated heart ODC was strongly inhibited. The addition of 2 mM DTT in the assaying buffer removed the ODC inactivation. 3. The inhibited ODC had an eluition profile similar to active ODC when chromatographed on a Sephacryl S-200 column; moreover, the ODC activity recovered after a thiol affinity chromatography as unbound fraction, was two times increased in the t-butylhydroperoxide perfused hearts in comparison to control. 4. The hearts perfused with 1 mM acetylcysteine after 15 min of perfusion with t-butylhydroperoxide recovered almost completely the initial ODC activity.

用100微摩尔叔丁基过氧化氢对离体大鼠心脏灌注15分钟，会导致组织谷胱甘肽/氧化型谷胱甘肽比值降低75%。2. 输注叔丁基过氧化氢后，异丙肾上腺素刺激的心脏鸟氨酸脱羧酶（ODC）受到强烈抑制。在测定缓冲液中加入2毫摩尔二硫苏糖醇（DTT）可消除ODC的失活。3. 当在Sephacryl S - 200柱上进行色谱分析时，受抑制的ODC具有与活性ODC相似的洗脱图谱；此外，经硫醇亲和色谱作为未结合部分回收后，叔丁基过氧化氢灌注心脏中的ODC活性与对照相比增加了两倍。4. 在叔丁基过氧化氢灌注15分钟后，用1毫摩尔乙酰半胱氨酸灌注的心脏几乎完全恢复了初始ODC活性。