Guarnieri C, Rossoni Caldarera C, Muscari C, Flamigni F, Caldarera C M
Ital J Biochem. 1982 Nov-Dec;31(6):404-11.
Cardiac ODC was prepared from rats after the injection of isoproterenol and was partially purified by thiol-affinity chromatography. This enzymatic preparation incubated with GSSG was inactivated in a concentration dependent process. The inactivated enzyme could be reactivated by the addition of various reducing compounds, including GSH. Kinetic studies on GSSG-inactivated ODC revealed that the enzyme was inhibited by a partially-non competitive mechanism.
心脏鸟氨酸脱羧酶（ODC）是在给大鼠注射异丙肾上腺素后制备的，并通过硫醇亲和色谱法进行部分纯化。这种酶制剂与氧化型谷胱甘肽（GSSG）一起孵育时，会在浓度依赖性过程中失活。失活的酶可以通过添加各种还原化合物（包括谷胱甘肽（GSH））而重新激活。对GSSG失活的ODC进行的动力学研究表明，该酶受到部分非竞争性机制的抑制。
