Xiao Xiangyu, Hao Xiangqi, Chen Bo, Zhou Pei, Li Shoujun
Guangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Guangdong Technological Engineering Research Center for Pets, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Vet Sci. 2022 Dec 26;10(1):14. doi: 10.3390/vetsci10010014.
Respiratory tract and intestinal diseases are common threats to feline health. Coinfection with multiple pathogens is not rare among clinical infectious cases. Rapid diagnosis of these coinfections is of great significance for timely and effective clinical treatment. In this study, two novel multiplex polymerase chain reactions (mPCRs) were established for simultaneous detection of four pathogens associated with the feline intestinal tract (feline coronavirus (FCoV), feline astrovirus (FeAstV), feline panleukopenia virus (FPV) and feline kobuvirus (FeKoV)) and five pathogens associated with the respiratory tract (feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), feline leukemia virus (FeLV), () and influenza A virus (IAV)). The results of sensitivity analysis revealed that the detection limits for FeKoV, FPV, FeAstV, FCoV, IAV, , FeLV, FHV-1 and FCV were 10, 10, 10, 10, 10, 10, 10, 10 and 10 copies/µL, respectively. Moreover, the specificity of the two mPCRs was high. When the two mPCRs were applied to clinical samples, the assay worked well. In conclusion, we established two mPCR methods that provide an excellent tool for the diagnosis and monitoring of pathogens associated with the feline respiratory and intestinal tracts.
呼吸道和肠道疾病是对猫健康的常见威胁。在临床感染病例中，多种病原体的合并感染并不罕见。快速诊断这些合并感染对于及时有效的临床治疗具有重要意义。在本研究中，建立了两种新型多重聚合酶链反应（mPCR），用于同时检测与猫肠道相关的四种病原体（猫冠状病毒（FCoV）、猫星状病毒（FeAstV）、猫泛白细胞减少症病毒（FPV）和猫杯状病毒（FeKoV））以及与呼吸道相关的五种病原体（猫杯状病毒（FCV）、猫疱疹病毒1型（FHV-1）、猫白血病病毒（FeLV）、（此处原文缺失一种病原体名称）和甲型流感病毒（IAV））。敏感性分析结果显示，FeKoV、FPV、FeAstV、FCoV、IAV、（此处原文缺失一种病原体名称）、FeLV、FHV-1和FCV的检测限分别为10、10、10、10、10、10、10、10和10拷贝/微升。此外，这两种mPCR的特异性很高。当将这两种mPCR应用于临床样本时，检测效果良好。总之，我们建立了两种mPCR方法，为猫呼吸道和肠道相关病原体的诊断和监测提供了一个极好的工具。
