College of Animal Science, Anhui Science and Technology University, Chuzhou, 233100, China.
Anhui Province Key Laboratory of Animal Nutrition Regulation and Health, Chuzhou, 233100, China.
Sci Rep. 2023 Jan 20;13(1):1164. doi: 10.1038/s41598-023-28503-5.
Ionomics-metabolomics association analysis is a novel method to elucidating the potential mechanisms underlying the effects of dietary copper on the overall health parameters of suckling piglets model. Few studies have elucidated the relationship between the changes of ionic and metabolic homeostasis responses to dietary copper level. The growth performance data was obtained from 180 suckling piglets which access to different copper levels: 6 (low copper diet, LC), 20 (control diet, CON), and 300 (high copper diet, HC) mg·kg copper (based on diet, supplementation from CuSO), and offered ad libitum from d 14 until weaning at 40 d of age. Dietary high level copper (300 mg·kg) increased the ADG and ADFI during d 14 to 28 of piglets. Six elements (Mg, Na, K, P, Cu, and Mn) concentrations significantly changes in hair among the three treatment diets. The significant increased concentrations of Na and K, and decreased concentration of Mg and Mn in 300 mg·kg than 20 mg·kg copper diet was observed. In current study, with the increase in copper level from 20 to 300 mg·kg in diet, the correlation between hair Na, K and Cu, Mn, Zn vanish. Hair Na and K were positively correlated with serum total antioxidant capacity (T-AOC) and negatively correlated with tumor necrosis factor-α (TNF-α). The hair Cu was negatively correlated with serum malondialdehyde (MDA), total bile acid (TBA). The fecal Cu was positively correlated with serum growth hormone (GH). The results suggested that the average daily gain (ADG) in 6 mg·kg copper diet and the average daily feed intake (ADFI) in 20 mg·kg copper diet were decreased than 300 mg·kg copper diet during d 14 to 28 and the ADG was decreased in 6 and 20 mg·kg copper diets in d 29 to 40 of piglets. Dietary 20 mg·kg copper maintain ion homeostasis due to increase the number of positive correlations between macroelements-microelements in hair and serum. Significantly changed Na, K, Mg, Mn and Cu concentrations in hair can reflect the adverse effects of dietary 300 mg·kg copper of suckling piglets. We believe our results may benefit people to gain a better understanding of the ion interactions and metabolic homeostasis of heavy metal elements that are critical to human and animal health.
离子组学-代谢组学关联分析是一种阐明膳食铜对哺乳仔猪模型整体健康参数影响潜在机制的新方法。很少有研究阐明了离子和代谢动态平衡对膳食铜水平变化的反应之间的关系。生长性能数据来自 180 头哺乳仔猪，它们接触到不同的铜水平：6（低铜饮食，LC）、20（对照饮食，CON）和 300（高铜饮食，HC）mg·kg 铜（基于饮食，补充 CuSO），并在 14 天至 40 天断奶期间自由采食。高铜饮食（300 mg·kg）在仔猪 14 至 28 天期间增加了 ADG 和 ADFI。在三种处理饮食中，头发中的 6 种元素（Mg、Na、K、P、Cu 和 Mn）浓度显著变化。与 20 mg·kg 铜饮食相比，300 mg·kg 铜饮食中 Na 和 K 的浓度显著增加，而 Mg 和 Mn 的浓度降低。在本研究中，随着饮食中铜水平从 20 增加到 300 mg·kg，头发中 Na、K 与 Cu、Mn、Zn 的相关性消失。头发中的 Na 和 K 与血清总抗氧化能力（T-AOC）呈正相关，与肿瘤坏死因子-α（TNF-α）呈负相关。头发中的 Cu 与血清丙二醛（MDA）、总胆汁酸（TBA）呈负相关。粪便中的 Cu 与血清生长激素（GH）呈正相关。结果表明，6 mg·kg 铜饮食的平均日增重（ADG）和 20 mg·kg 铜饮食的平均日采食量（ADFI）在 14 至 28 天期间低于 300 mg·kg 铜饮食，6 和 20 mg·kg 铜饮食在仔猪 29 至 40 天期间的 ADG 降低。20 mg·kg 铜的饮食维持离子动态平衡，因为头发和血清中宏观元素-微量元素之间的正相关数量增加。头发中显著变化的 Na、K、Mg、Mn 和 Cu 浓度可以反映哺乳仔猪 300 mg·kg 铜饮食的不良影响。我们相信，我们的研究结果可以使人们更好地了解对人类和动物健康至关重要的重金属元素的离子相互作用和代谢动态平衡。
