Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, Suita 565-0871, Japan.
Biomolecules. 2022 Dec 30;13(1):79. doi: 10.3390/biom13010079.
To improve current infertility treatments, it is important to understand the pathophysiology of implantation failure. However, many molecules are involved in the normal biological process of implantation and the roles of each molecule and the molecular mechanism are not fully understood. This review highlights the hemagglutinating virus of Japan (HVJ; Sendai virus) envelope (HVJ-E) vector, which uses inactivated viral particles as a local and transient gene transfer system to the murine uterus during the implantation period in order to investigate the molecular mechanism of implantation. In vivo screening in mice using the HVJ-E vector system suggests that signal transducer and activator of transcription-3 (Stat-3) could be a diagnostic and therapeutic target for women with a history of implantation failure. The HVJ-E vector system hardly induces complete defects in genes; however, it not only suppresses but also transiently overexpresses some genes in the murine uterus. These features may be useful in investigating the pathophysiology of implantation failure in women.
为了改善当前的不孕治疗方法，了解着床失败的病理生理学非常重要。然而，许多分子参与了着床的正常生物学过程，每个分子的作用及其分子机制尚未完全阐明。本综述重点介绍了日本血凝病毒（HVJ；仙台病毒）包膜（HVJ-E）载体，该载体利用失活的病毒颗粒作为局部和短暂的基因转移系统，在着床期将基因转移到小鼠子宫内，以研究着床的分子机制。在小鼠体内使用 HVJ-E 载体系统进行的筛选表明，信号转导和转录激活因子 3（Stat-3）可能是着床失败史女性的诊断和治疗靶点。HVJ-E 载体系统几乎不会引起基因的完全缺失；然而，它不仅抑制而且短暂地过表达了小鼠子宫中的一些基因。这些特征可能有助于研究女性着床失败的病理生理学。
