School of Biology, Food and Environment, Hefei University, Hefei 230601, China.
Genes (Basel). 2023 Jan 15;14(1):226. doi: 10.3390/genes14010226.
The main cause of Parkinson's disease (PD) remains unknown and the pathologic changes in the brain limit rapid diagnosis. Herein, differentially expressed genes (DEGs) in the Gene Expression Omnibus (GEO) database (GSE8397 and GSE22491) were assessed using linear models for microarray analysis (limma). () was the only common gene differentially down-regulated in lateral substantia nigra (LSN), medial substantia nigra (MSN) and blood. Additionally, DEGs between high and low in GSE99039 were picked out and then uploaded to the Database for Annotation, Visualization and Integrated Discovery (DAVID) for gene ontology (GO) functional annotation analysis. GO analysis displayed that these DEGs were mainly enriched in oxygen transport, myeloid cell development and gas transport (biological process (BP)); hemoglobin complex, haptoglobin-hemoglobin complex and cortical cytoskeleton (cellular component (CC)); and oxygen transporter activity, haptoglobin binding and oxygen binding (molecular function (MF)). Receiver operating characteristic (ROC) curve analysis showed had good diagnostic accuracy and increased the area under the curve (AUC) value when combined with other biomarkers. Consistently, intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropy-ridi-ne (MPTP) in C57BL/6J mice reduced mRNA expression in both substantia nigra and blood compared to the control group. Thus, may serve as a candidate biomarker for PD diagnosis.
帕金森病 (PD) 的主要病因仍不清楚，大脑的病理变化限制了快速诊断。在此，使用线性模型对基因表达综合数据库 (GEO) 中的差异表达基因 (DEGs) 进行了评估 (GSE8397 和 GSE22491)。()是在外侧黑质 (LSN)、内侧黑质 (MSN) 和血液中唯一共同下调的差异基因。此外，从 GSE99039 中挑选出 高 和 低 之间的差异基因，然后上传到数据库进行注释、可视化和综合发现 (DAVID) 进行基因本体 (GO) 功能注释分析。GO 分析显示，这些 DEGs 主要富集在氧气运输、髓样细胞发育和气体运输（生物过程 (BP))；血红蛋白复合物、触珠蛋白-血红蛋白复合物和皮质细胞骨架 (CC)；以及氧气转运蛋白活性、触珠蛋白结合和氧气结合（分子功能 (MF))。受试者工作特征 (ROC) 曲线分析表明，与其他生物标志物联合使用时，具有良好的诊断准确性，并增加了曲线下面积 (AUC) 值。同样，在 C57BL/6J 小鼠中腹腔注射 1-甲基-4-苯基-1,2,3,6-四氢吡啶 (MPTP) 后，与对照组相比，黑质和血液中的 mRNA 表达均降低。因此， 可能作为 PD 诊断的候选生物标志物。
