血红蛋白病的产前诊断：高效液相色谱法的潜在应用
Prenatal diagnosis of hemoglobinopathies: a potential application of high performance liquid chromatography.
作者信息
Alter B P, Stump D D
机构信息
Polly Annenberg Levee Hematology Center, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029.
出版信息
Hemoglobin. 1987;11(4):341-52. doi: 10.3109/03630268709042853.
High performance liquid chromatography was compared with carboxymethyl cellulose column chromatography data for analysis of globin chain composition and biosynthetic ratios in fetal blood. A formula was derived for mathematical correction for losses of small amounts of globin protein on the high performance liquid chromatography column. Radioactive globin chain ratios by high performance liquid chromatography are equivalent to those by carboxymethyl cellulose column chromatography, but only when carrier Hb A is added. We conclude that high performance liquid chromatography provides a suitable alternative to carboxymethyl cellulose columns, and has several advantages.
将高效液相色谱法与羧甲基纤维素柱色谱法的数据进行比较，以分析胎儿血液中的珠蛋白链组成和生物合成比率。推导了一个公式，用于对高效液相色谱柱上少量珠蛋白的损失进行数学校正。通过高效液相色谱法得到的放射性珠蛋白链比率与通过羧甲基纤维素柱色谱法得到的比率相当，但前提是添加载体血红蛋白A。我们得出结论，高效液相色谱法是羧甲基纤维素柱的合适替代方法，并且具有几个优点。
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