Congote L F
Blood. 1981 Feb;57(2):353-60.
Reversed-phase high pressure liquid chromatography (HPLC) was used for the separation of the alpha, beta, (A)gamma and (G)gamma chains from human blood samples. The alpha and beta chains were normally eluted close together, but their separation was improved by coupling 2 or 3 columns in series, or by increasing the temperature of the columns. This method has been applied for the determination of beta/gamma ratios in blood samples obtained at fetoscopy from normal pregnancies and fetuses at risk for beta-thalassemia. The values obtained by high pressure chromatography were similar but slightly lower than those found by carboxymethyl cellulose (CMC) chromatography. The average (G)gamma/(A)gamma ratio of the chains labeled after a 2-hr pulse with [3H] leucine was almost identical to the actual (G)gamma/(A)gamma measured by absorbance at 280 nm, indicating a constant rate of synthesis and accumulation of both globin chains in the first trimester fetus.
反相高效液相色谱法（HPLC）用于从人血样本中分离α、β、（A）γ和（G）γ链。α链和β链通常洗脱时靠得很近，但通过串联2或3根柱子，或提高柱温可改善它们的分离效果。该方法已应用于测定通过胎儿镜检查从正常妊娠和有β地中海贫血风险的胎儿获得的血样中的β/γ比值。高压色谱法得到的值与羧甲基纤维素（CMC）色谱法得到的值相似，但略低。用[3H]亮氨酸脉冲标记2小时后标记的链的平均（G）γ/（A）γ比值几乎与在280nm处通过吸光度测量的实际（G）γ/（A）γ比值相同，表明在孕早期胎儿中两条珠蛋白链的合成和积累速率恒定。
