Zhang Qinqiu, Wang Zhuwei, Li Yinglu, Liu Xinzhi, Liu Lang, Yan Jing, Hu Xinjie, Qin Wen
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Foods. 2023 Jan 13;12(2):395. doi: 10.3390/foods12020395.
Okra has received extensive attention due to its high nutritional value and remarkable functional characteristics, but postharvest diseases have severely limited its application. It is important to further explore the methods and potential methods to control the postharvest diseases of okra. In this study, is the major pathogen that causes okra anthracnose, which can be isolated from naturally decaying okra. The pathogenicity of against okra was preliminarily verified, and the related biological characteristics were explored. At the same time, an observational study was conducted to investigate the in vitro antifungal effect of thymol edible coating (TKL) on . After culturing at 28 °C for 5 days, it was found that TKL showed an obvious growth inhibition effect on . The concentration for 50% of the maximal effect was 95.10 mg/L, and the minimum inhibitory concentration was 1000 mg/L. In addition, it was found that thymol edible coating with a thymol concentration of 100 mg/L (TKL100) may cause different degrees of damage to the cell membrane, cell wall, and metabolism of , thereby inhibiting the growth of hyphae and causing hyphal rupture. Refer to the results of the in vitro bacteriostatic experiment. Furthermore, the okra was sprayed with TKL100. It was found that the TKL100 coating could significantly inhibit the infection of to okra, reduce the rate of brown spots and fold on the okra surface, and inhibit mycelium growth. In addition, the contents of total phenols and flavonoids of okra treated with TKL100 were higher than those of the control group. Meanwhile, the activities of phenylalaninammo-nialyase, cinnamic acid-4-hydroxylase, and 4-coumarate-CoA ligase in the lignin synthesis pathway were generally increased, especially after 6 days in a 28 °C incubator. The lignin content of TKL-W was the highest, reaching 65.62 ± 0.68 mg/g, which was 2.24 times of that of CK-W. Therefore, TKL may promote the synthesis of total phenols and flavonoids in okra, then stimulate the activity of key enzymes in the lignin synthesis pathway, and finally regulate the synthesis of lignin in okra. Thus, TKL could have a certain controlling effect on okra anthracnose.
秋葵因其高营养价值和显著的功能特性而受到广泛关注，但采后病害严重限制了其应用。进一步探索控制秋葵采后病害的方法和潜在方法很重要。在本研究中，[具体病菌名称未给出]是导致秋葵炭疽病的主要病原菌，可从自然腐烂的秋葵中分离得到。初步验证了[具体病菌名称未给出]对秋葵的致病性，并探索了其相关生物学特性。同时，进行了一项观察性研究，以研究百里香酚可食性涂层（TKL）对[具体病菌名称未给出]的体外抗真菌效果。在28℃培养5天后，发现TKL对[具体病菌名称未给出]有明显的生长抑制作用。半数最大效应浓度为95.10mg/L，最低抑菌浓度为1000mg/L。此外，发现百里香酚浓度为1mg/L的百里香酚可食性涂层（TKL100）可能对[具体病菌名称未给出]的细胞膜、细胞壁和代谢造成不同程度的损伤，从而抑制菌丝生长并导致菌丝破裂。参考体外抑菌实验结果。此外，用TKL100对秋葵进行喷洒处理。发现TKL100涂层可显著抑制[具体病菌名称未给出]对秋葵的侵染，降低秋葵表面的褐斑率和褶皱率，并抑制菌丝生长。此外，用TKL100处理的秋葵中总酚和黄酮类化合物的含量高于对照组。同时，木质素合成途径中苯丙氨酸解氨酶、肉桂酸-4-羟化酶和4-香豆酸-CoA连接酶的活性普遍升高，尤其是在28℃培养箱中培养6天后。TKL-W的木质素含量最高，达到65.62±0.68mg/g，是CK-W的2.24倍。因此，TKL可能促进秋葵中总酚和黄酮类化合物的合成，进而刺激木质素合成途径中关键酶的活性，最终调节秋葵中木质素合成。因此，TKL对秋葵炭疽病可能有一定的防治效果。
