Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2023 Jan 4;24(2):906. doi: 10.3390/ijms24020906.
Roots are essential for plant growth, and studies on root-related genes, exemplified by (), have mainly concentrated on model organisms with less emphasis on the function of these genes in woody plants. Here, we report that overexpression of the gene from hybrid () in leads to significant morphological changes in both the aerial and subterranean organs. In the aerial parts, overexpression of results in the production of ectopic floral meristems and leaves, possibly via the ectopic activation of and . In addition, in the root, overexpression of alters root apical meristem morphology, leading to a curled and shortened primary root. Importantly, these abnormal phenotypes in the aerial and subterranean organs caused by constitutive ectopic expression of mimic the observed phenotypes when overexpressing and in , respectively. Taken together, we propose that the 5 gene, originating from the Magnoliaceae plant , is a functional homolog of the gene from , while showing the highest degree of sequence similarity with its ortholog, . Our study provides insight into the potential role of in the development of both the shoot and root.
根对于植物的生长至关重要，以 ()为例的与根相关的基因研究主要集中在模式生物上，而对木本植物中这些基因的功能关注较少。在这里，我们报告了杂种 ()中的 基因的过表达导致了 ()在空中和地下器官中都有显著的形态变化。在空中部分， 过表达导致异位花分生组织和叶片的产生，可能是通过 和 的异位激活。此外，在根中， 过表达改变了根顶端分生组织的形态，导致主根卷曲和缩短。重要的是，由组成型异位表达 引起的地上和地下器官的这些异常表型分别模拟了在 中过表达 和 时观察到的表型。总之，我们提出来自木兰科植物的 5 基因是 基因的功能同源物，而与它的直系同源物 具有最高的序列相似性。我们的研究为 基因在芽和根发育中的潜在作用提供了深入了解。
