State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2024 Jun 26;25(13):6968. doi: 10.3390/ijms25136968.
Chloroplasts is the site for photosynthesis, which is the main primary source of energy for plants. Golden2-like (GLK) is a key transcription factor that regulates chloroplast development and chlorophyll synthesis. However, most studies on genes are performed in crops and model plants with less attention to woody plants. In this study, we identified the and genes in the woody plant hybrid, and they are specifically expressed in green tissues. We showed that overexpression of the gene improves rosette leaf chlorophyll content and induces ectopic chlorophyll biogenesis in primary root and petal vascular tissue in . Although these exhibit a late-flowering phenotype, transgenic lines accumulate more biomass in vegetative growth with improved photochemical quenching (qP) and efficiency of photosystem II. Taken together, we verified a conserved and ancient mechanism for regulating chloroplast biogenesis in hybrid and evaluated its effect on photosynthesis and rosette biomass accumulation in the model plant .
叶绿体是光合作用的场所，是植物主要的能量来源。类金色素体 2（GLK）是一种关键的转录因子，调控叶绿体发育和叶绿素合成。然而，大多数关于基因的研究都是在农作物和模式植物中进行的，对木本植物的关注较少。在这项研究中，我们鉴定了木本植物杂种中的和基因，它们在绿色组织中特异性表达。我们表明，过表达基因可提高拟南芥莲座叶的叶绿素含量，并诱导主根和花瓣维管束组织中的异位叶绿素生物发生。尽管这些植物表现出晚花表型，但转基因株系在营养生长中积累了更多的生物量，并且具有改进的光化学猝灭（qP）和光系统 II 的效率。总的来说，我们验证了调控杂种叶绿体生物发生的保守而古老的机制，并评估了它对模式植物光合作用和莲座叶生物量积累的影响。
