WUSCHEL 相关同源框 5a（PtoWOX5a）参与杨树不定根的发育。

The WUSCHEL-related homeobox 5a (PtoWOX5a) is involved in adventitious root development in poplar.

机构信息

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100091, China.

出版信息

Tree Physiol. 2018 Jan 1;38(1):139-153. doi: 10.1093/treephys/tpx118.

DOI:10.1093/treephys/tpx118

PMID:29036435

Abstract

Adventitious rooting is an essential step in vegetative propagation. Currently, the mechanism that regulates adventitious root (AR) development in woody plants is poorly understood. This work demonstrates that Populus tomentosa WUSCHEL-related homeobox 5a (PtoWOX5a) transcription factor is involved in AR development in poplar. PtoWOX5a was specifically expressed in the AR tip and lateral root tip during AR and lateral root regeneration from the stem segment. Phenotypic complementation experiments indicated that the PtoWOX5a can functionally complement AtWOX5 in quiescent center (QC) cells. Overexpression of PtoWOX5a introduces significant developmental phenotypes in roots and leaves, such as increased AR number, decreased AR length, swollen AR tip and lateral root tip, and decreased leaf number and area. The conserved mechanism of D-type cyclins (CYCD) repression mediated by WOX5 was confirmed in poplar. The co-expression network of PtWOX5a was constructed, which provided clues to reveal the molecular mechanism of PtoWOX5a in AR development in poplar. Taken together, our results suggest that the PtoWOX5a is involved in AR development though cooperating with a series of functional genes.

摘要

不定根的形成是营养繁殖中的一个重要步骤。目前，木质植物不定根（AR）发育的调控机制还不清楚。本研究表明，毛白杨 WUSCHEL 相关同源框 5a（PtoWOX5a）转录因子参与杨树不定根的发育。在 AR 和侧根从茎段再生过程中，PtoWOX5a 特异性地在 AR 顶端和侧根顶端表达。表型互补实验表明，PtoWOX5a 可以在静止中心（QC）细胞中功能性地补充拟南芥 AtWOX5。过表达 PtoWOX5a 会导致根和叶出现明显的发育表型，例如增加不定根的数量、减少不定根的长度、不定根和侧根顶端肿胀以及叶片数量和面积减少。在杨树中证实了 WOX5 介导的 D 型细胞周期蛋白（CYCD）抑制的保守机制。构建了 PtWOX5a 的共表达网络，为揭示 PtoWOX5a 在杨树不定根发育中的分子机制提供了线索。综上所述，我们的结果表明，PtoWOX5a 通过与一系列功能基因的合作参与不定根的发育。