BSA-Seq 与 RNA-Seq 整合揭示了玉米中一个新的 Fasciated Ear5 突变体。

Integrating BSA-Seq with RNA-Seq Reveals a Novel Fasciated Ear5 Mutant in Maize.

机构信息

National Key Laboratory of Wheat and Maize Crop Science, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.

The Shennong Laboratory, Zhengzhou 450002, China.

出版信息

Int J Mol Sci. 2023 Jan 7;24(2):1182. doi: 10.3390/ijms24021182.

DOI:10.3390/ijms24021182

PMID:36674701

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9867142/

Abstract

Increasing grain yield is required to meet the rapidly expanding demands for food, feed, and fuel. Inflorescence meristems are central to plant growth and development. However, the question concerning whether inflorescence development can be regulated to improve grain yield remains unclear. Here, we describe a naturally occurring single recessive mutation called that can increase grain yield in maize. Using bulk segregant analysis sequencing (BSA-seq), the candidate region was initially mapped to a large region on chromosome 4 (4.68 Mb-11.26 Mb). Transcriptome sequencing (RNA-seq) revealed a total of 1246 differentially expressed genes (DEGs), of which 835 were up-regulated and 411 were down-regulated. Further analysis revealed the enrichment of DEGs in phytohormone signal transduction. Consistently, phytohormone profiling indicated that auxin (IAA), jasmonic acid (JA), ethylene (ETH), and cytokinin (CK) levels increased significantly, whereas the gibberellin (GA) level decreased significantly in . By integrating BSA-seq with RNA-seq, we identified as the most likely candidate gene. Our results provide valuable insight into this new germplasm resource and the molecular mechanism underlying fasciated ears that produce a higher kernel row number in maize.

摘要

为了满足粮食、饲料和燃料需求的快速增长，提高粮食产量是必要的。花序分生组织是植物生长和发育的核心。然而，花序发育是否可以调控以提高粮食产量的问题仍不清楚。在这里，我们描述了一种自然发生的隐性单突变体，称为，它可以提高玉米的粒产量。利用大量分离群体分析测序（BSA-seq），候选区域最初被映射到 4 号染色体上的一个大区域（4.68 Mb-11.26 Mb）。转录组测序（RNA-seq）共发现了 1246 个差异表达基因（DEGs），其中 835 个上调，411 个下调。进一步分析表明，DEGs 在植物激素信号转导中富集。一致地，植物激素分析表明，生长素（IAA）、茉莉酸（JA）、乙烯（ETH）和细胞分裂素（CK）水平显著增加，而赤霉素（GA）水平在中显著降低。通过将 BSA-seq 与 RNA-seq 相结合，我们鉴定出为最可能的候选基因。我们的研究结果为这种新的种质资源以及玉米中产生更高的籽粒行数的束状穗的分子机制提供了有价值的见解。